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Analysis of the interaction Moniliophthora perniciosa x 'Micro-Tom' tomato: non-host type resistance, effects on the development and role of phytohormones in pathogenesis

Grant number: 17/17000-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2017
Effective date (End): October 31, 2020
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Antonio Vargas de Oliveira Figueira
Grantee:Eder Marques da Silva
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:16/10498-4 - Investigation of strategies of adaptation to the pathogenic life style of fungi from the Moniliophthora genus at various levels of biological organizations: species, biotypes, and geographic lineages, AP.TEM

Abstract

The analysis of the Moniliophthora perniciosa x MT interaction showed some evidences to be a case of a broken non-host resistance (NHR) by the S-biotype in tomato, but not by the C-biotype. The limited rate of MT penetration by basidiospores with emerging mycelia suggest a little developed pathogenic interaction between the S-biotype and MT. Molecular features of NHR will be evaluated by RNA-seq and NHR candidate gene expression analysis in the S- and C-biotype x MT interactions, together with histological observations of early penetration events. Recent reports in other pathosystems suggest that, besides effector proteins, pathogens can employ small RNAs (sRNAs) to directly manipulate the plant defense system, and, therefore, identification and expression analysis of sRNAs will be conducted during MT infection by M. perniciosa. sRNAs expression will be followed by RNA-seq during infection to investigate if this mechanism is also employed by M. perniciosa. The molecular analysis of non-host resistance (NHR), the potential involvement of small RNAs in the host x pathogen interaction and hormones in the defense response will be evaluated using the interaction ' Micro-Tom' (MT) tomato x basidiomycete Moniliophthora perniciosa (S-Biotype) as a model of the cacao (Theobroma cacao) witches´ broom pathosystem.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARSOTTINI, MARIO R. O.; PIRES, BARBARA A.; VIEIRA, MARIA L.; PEREIRA, JOSE G. C.; COSTA, PAULO C. S.; SANITA, JAQUELINE; CORADINI, ALESSANDRO; MELLO, FELLIPE; MARSCHALK, CIDNEI; SILVA, EDER M.; PASCHOAL, DANIELE; FIGUEIRA, ANTONIO; RODRIGUES, FABIO H. S.; CORDEIRO, ARTUR T.; MIRANDA, PAULO C. M. L.; OLIVEIRA, PAULO S. L.; SFORCA, MAURICIO L.; CARAZZOLLE, MARCELO F.; ROCCO, SILVANA A.; PEREIRA, GONCALO A. G. Synthesis and testing of novel alternative oxidase (AOX) inhibitors with antifungal activity against Moniliophthora perniciosa (Stahel), the causal agent of witches' broom disease of cocoa, and other phytopathogens. Pest Management Science, v. 75, n. 5, p. 1295-1303, MAY 2019. Web of Science Citations: 1.

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