Development of an immunosensor based on graphene for the detection of Enterotoxin A of Staphylococcus aureus in milk samples

• Grant number: 17/07288-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2017
• End date: October 31, 2019
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Ivana Cesarino
• Grantee: Giovanna da Silva Rocha
• Host Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 18/02382-1 - Evaluation of the immobilization of anti-SEA polyclonal antibodies on rGO derived from CVD graphene growth, BE.EP.IC

Abstract

Staphylococcus aureus is one of the microorganisms involved with food-borne diseases (FBD), mainly associated to the consumption of manually processed and prepared food. The intoxication related with the presence of S. aureus involves the production of staphylococcal enterotoxins (SEs), which SEA and SEB are the best characterized between the more than 20 existent types. Classified as superantigens, the SEs bind to the antigen-presenting cells (APCs) of the gastrointestinal epithelium, causing a series of immunological reactions that can culminate in inflammation. Nowadays the methods of SEs' detection demand time, specificity and pre-sample treatments, which can be a barrier to fast and effectiveness diagnostics. In this context, the development of an electrochemical immunosensor based on reduced graphene oxide (rGO) to optimize the methods of detection of SEs can be an interesting alternative, aiming the development of a technological platform that will be more sensitive and fast to determinate the enterotoxins. Also, the immunosensor will be based on immunological interactions between SEs and host APCs, increasing the needed specificity to detect SEA in artificially contaminated milk samples.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, MARTIN K. L.; LEAO, ALCIDES L.; SAIN, MOHINI; CESARINO, IVANA. A functionalized renewable carbon-based surface for sensor development. Journal of Solid State Electrochemistry, v. 25, n. 3, p. 1093-1099, MAR 2021. (17/07288-0)

ROCHA, GIOVANNA S.; SILVA, MARTIN K. L.; CESARINO, IVANA. Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples. MATERIALS, v. 13, n. 7, APR 2020. (17/07288-0)