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In vivo characterization of murine mesenchymal stem cells from bone marrow and compact bone in relation to its heterotopic hematopoietic niche generation potential

Grant number: 17/12663-5
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2017
Effective date (End): September 30, 2019
Field of knowledge:Biological Sciences - Biology
Principal Investigator:Dimas Tadeu Covas
Grantee:Rafaela Rossetti
Home Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil
Associated research grant:13/08135-2 - CTC - Center for Cell-Based Therapy, AP.CEPID

Abstract

The hematopoietic niche is the microenvironment that supports hematopoiesis regulating self-renewing, proliferation and hematopoietic stem cell differentiation. It is known that the endosteum region is important for the hematopoietic niche and that among the cell types that comprise this niche are the mesenchymal stem cells (MSC). Initially, bone marrow MSCs were described as perivascular located cells; however, later studies suggested that they might be present also in the endosteum region of young mice. In this way, a hypothesis is proposed for the existence of two distinct bone marrow MSC populations: one, comprised of osteochondroreticular cells, present in the endosteum and responsible for the generation and homeostasis of skeletal tissue during youth and the other formed by perisinusoidal cells in the perivascular niche, generating adipose tissue and playing a role in adulthood tissue repair. In this context, our research group previously observed MSCs, isolated from C57BL/6GFP mice bone marrow and compact bone, presented differences in its in vitro characteristics, thus, this proposition aims to compare these cells in relation to its potential to generate a heterotopic hematopoietic niche in vivo, post-transplant. The role of MSCs in the generation of a heterotopic hematopoietic niche is important either in a therapeutic aspect, contributing to an improvement of hematopoietic transplants, as for the generation of models for hematologic disease studies.