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The role of mast cells upon the in vitro osteogenic differentiation of the mesenchymal stromal cells from the bone marrow of mice

Grant number: 17/07095-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2017
Effective date (End): September 30, 2019
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Sandra Helena Penha de Oliveira
Grantee:Maria Carolina Linjardi de Sousa
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
Associated research grant:15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM

Abstract

Bone tissue is formed by some important cell types, such as: osteoblasts, osteoclasts and osteocytes. Osteoblasts are bone-forming cells, which synthesize and secrete collagen fibers and other organic components necessary for the formation of extracellular bone matrix, in particular collagen I, bone sialoprotein, osteocalcin and osteopontin. The osteogenesis process is divided into three stages (proliferation, phenotypic impairment, and matrix synthesis). It involves many factors for an ostoprogenitor cell to differentiate as the involvement of growth factors, cytokines and chemokines. Many of these factors may come from the resident cells themselves located near the bone tissue that contribute, or not, to the progenitor cells to assume specific phenotypes. Studies have revealed the possibility that mast cells have the ability to regulate bone turnover through their preformed products since these cells are strategically located around the periosteal surface, thus being in close anatomical proximity to periosteal osteoblasts. Thus, understanding the role of mast cells in the osteoblast differentiation of osteoblasts, as well as the mechanisms involved in this process, is of extreme importance in order to better understand the participation of these cells as well as their mediators in the development of bone disorders for example, in the osteoporosis. Thus, in the present study, we propose to evaluate the role of mast cells on the osteoblastic differentiation of mesenchymal stromal cells from the bone marrow of mice in order to investigate possible mechanisms involved in this process.