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Therapeutic potential of low-level laser in the brain of aged rats

Grant number: 17/16443-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2017
Status:Discontinued
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Sérgio Gomes da Silva
Grantee:Fabrízio dos Santos Cardoso
Home Institution: Pró-Reitoria Acadêmica. Universidade de Mogi das Cruzes (UMC). Campus da Sede Mogi das Cruzes. Mogi das Cruzes , SP, Brazil
Associated scholarship(s):19/24136-5 - Cytochrome oxidase activity in the brain of aged rats exposed to low level laser therapy: a quantitative histochemical study, BE.EP.DR

Abstract

Around the world, the proportion of people over 60 is increasing as faster as any other age group. It is estimated that by 2025 the population of the elderly will be 1.2 billion people. In Brazil, it will be approximately 30 million. However, longevity has been accompanied by an increase in cases of neurological diseases associated with aging, such as Alzheimer's disease, Parkinson's disease and others. Thus, the search for new forms of therapeutic treatments and intervention has been of great interest to the medical community. In this context, low-level laser therapy (LLLT) has become very promising, mainly to it neuroprotective effects. Based on this, the purpose of the present study will be to test the therapeutic potential of LLLT in the brain of aged rats. For that, we will analyze in rats with 2 and 20 month-old submitted to LLLT during 28 days: (1) the cognitive performance of rats in novel object recognition test, Barnes maze, 8-arm radial maze, elevated plus maze, and inhibitory avoidance; (2) the neuronal morphology in cerebral córtex and hippocampal formation by the Cox-Golgi method; (3) the brain expression of brain-derived neurotrophic fator (BDNF) and intracellular signaling pathways linked, such as extracellular signal-regulated kinase (ERK), protein kinase B (Akt), cyclic AMP-response element-binding protein (CREB), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38). Our hypothesis is that LLLT results in increased cognitive performance in elderly rats, and also promotes increased dendritic arborization and increased activation of intracellular proteins linked to survival and proliferation (such as ERK, Akt and CREB) or decreased activation of intracellular proteins associated with death (such as JNK and p38). (AU)