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Forced expression effect of GM-CSF and M-CSF genes in ischemic skeletal muscle

Grant number: 17/17588-1
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2018
Effective date (End): December 31, 2019
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal researcher:Sang Won Han
Grantee:Yuan-Chi Teng
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated research grant:15/20206-8 - Modulation of monocytes, macrophages and pericytes by the colony stimulating factor genes to treat murine limb ischemia, AP.TEM

Abstract

The proposed thematic project aims to study the effect of colony stimulating factors (CSFs) on the modulation of macrophages, monocytes and pericytes, since we and other groups have seen that CSFs have the activity of forming new vessels and remodeling blood vessels. In addition, we observed the anti-fibrotic and pro-myogenic activities, which is not a consensus with other groups. Thus, it is extremely important to analyze the alteration of the expression pattern of genes that contribute to angiogenesis, fibrogenesis and myogenesis after transfection with the CSF genes during various post-ischemic times. In addition, the effect of overexpression of CSFs on pericytes is unknown. Today these cells are considered as mesenchymal stem cells with ability to differentiate into several cell types and, therefore, are very important for tissue repair.Based on these observations, the postdoctoral researcher will work to answer the following objectives: 1) To analyze the main genes involved in angiogenesis, myogenesis and fibrogenesis by RT-qPCR after transfection of ischemic and normoxic skeletal muscle with GM-CSF or M-CSF expressing vector. 2) To study the effects of GM-CSF and M-CSF genes on the activation and differentiation of pericytes.