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Effect of Pregnancy-Associated Plasma Protein- A (PAPP-A) about profile and lipid metabolism of bovine oocytes matured in vitro

Grant number: 17/19679-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2017
Effective date (End): November 30, 2018
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Anthony César de Souza Castilho
Grantee:Thaisy Tino Dellaqua
Home Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
Associated research grant:13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression, AP.JP


Insulin-like growth factor (IGF) along with its binding protein (IGFBP), participates in lipid metabolism and adiposity regulation. A good regulator for this group is Pregnancy-Associated Plasma Protein-A (PAPP-A), which is capable of breaking the bonds between IGF and its binding proteins, while increasing the bioavailability of free IGF, leading to a reduction in the amount of lipids due to the increase in the rate of ²-oxidation. Many studies have already investigated the action of exogenous IGF-I on in vitro maturation (IVM), however, little was known about the effects of PAPP-A addition during this stage. Recent data obtained by our group report that the addition of 100 ·g/mL of PAPP-A during IVM has shown positive effects for embryo quality. Thus, it is of great importance to assess the addition or modulation of the IGF composition and its availability. In this context, the objective of this research project is to investigate the effects of the addition of 100 ·g/mL PAPP-A during IVM of cumulus oocyte complexes (COCs) from slaughterhouse ovaries on: 1) the lipid content of oocytes using Sudan-Black B coloration; 2) the membrane phospholipid profile of COCs, through mass spectrometry analysis (MALDI-MS); 3) the expression pattern of genes related to the lipid profile. (AU)

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