Metabolism of the brown adipose tissue in animal models of insulin resistance: Goto-Kakizaki and obese Wistar rats

• Grant number: 16/14529-1
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: December 01, 2017
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Sandro Massao Hirabara
• Grantee: Tamires Duarte Afonso Serdan
• Host Institution: Centro de Ciências Biológicas e da Saúde. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
• Associated scholarship(s): 18/18815-4 - Brite/beige adipocytes: a potential tool against obesity and diabetes?, BE.EP.DD

Abstract

Overweight and obesity are related to several disorders, such as insulin resistance, chronic inflammation, Type 2 Diabetes Mellitus, cardiovascular diseases, dyslipidemia, metabolic syndrome, and cancer. Brown Adipose Tissue (BAT) has been proposed as a potential intervention for obesity because its high metabolic capacity to dissipate energy as heat without ATP synthesis, enhancing energy expenditure. In addition, BAT is responsive to the insulin and it helps to improve glycemic homeostasis, but the precise mechanisms are not completely understood yet. The aims of this project are to characterize the role of BAT in three animal models of insulin resistance: obese (Wistar rats submitted to a high-fat diet or high-fat plus high-sugar diet) and non-obese (Goto-Kakizaki rats, genetically predisposed to develop IR) animals. We will investigate the following parameters: 1) BAT metabolism in isolated mitochondria; 2) secretion of molecules by BAT (batokines), and 3) expression of proteins involved in the insulin-signaling pathway (IRS-1, Akt, GSK-3, PKC-q, e PI3-K). Therefore, this project will be important to characterize the BAT adipose tissue function and its metabolic influence in the insulin resistant state in different animal models (obese and non- obese rats). Elucidation of the BAT participation in these models is primordial, since it can help to direct new studies and strategies for improving glycemic homeostasis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SERDAN, TAMIRES DUARTE AFONSO; MASI, LAUREANE NUNES; PEREIRA, JOICE NAIARA BERTAGLIA; RODRIGUES, LUIZ EDUARDO; ALECRIM, AMANDA LINS; SCERVINO, MARIA VITORIA MARTINS; DINIZ, VINICIUS LEONARDO SOUSA; DOS SANTOS, ALEF ARAGAO CARNEIRO; SOUSA FILHO, CELSO PEREIRA BATISTA; ALBA-LOUREIRO, TATIANA CAROLINA; et al. Impaired brown adipose tissue is differentially modulated in insulin-resistant obese wistar and type 2 diabetic Goto-Kakizaki rats. BIOMEDICINE & PHARMACOTHERAPY, v. 142, OCT 2021. (18/09868-7, 18/18815-4, 19/25936-5, 16/14529-1)