Impact of exposure to lead (Pb) on the expression profile of genes related to metal metabolism in occupationally exposed individuals

• Grant number: 16/24520-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2017
• End date: January 31, 2022
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Gustavo Rafael Mazzaron Barcelos
• Grantee: Marília Ladeira de Araújo
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated research grant: 13/06033-8 - Combining omics tools for predicting multivariate models in populations exposed to toxic metals, AP.JP
• Associated scholarship(s): 18/24643-1 - Impact of exposure to lead (Pb) on the expression profile of micro RNAs (miRNAs) in workers exposed to metal, BE.EP.DR

Abstract

There are wide variations in the concentrations of the biomarkers and toxicity related to lead (Pb), despite similar levels of exposure and this is hypothetically related genetic differences in the enzymes that metabolize metal. However, very little is known about the genetic effects on metabolism and toxicity induced by Pb. Therefore, this project aims to assess the gene-metal interactions in workers exosed to Pb in automotive batteries factory. For such, concentrations Pb will be determined in blood (Pb-B), plasma (Pb-P) and urine (U-Pb) by using a mass spectrometer with inductively coupled plasma (ICP-MS). The evaluation of genes related to the metabolism of the metal, VDR, SLC11A2, HFE, MT2a, ALAD, GCLM, GSS, GSR, GPx1, GGT1 and GSTP1, will be performed by qPCR (SYBR Green) and Western Blotting in order to verify the possible effects of metal exposure on expression of genes related to Pb-metabolism. Thus, it is expected that the results obtained will contribute in a better understanding of the several Pb-gene interactions.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO, MARILIA LADEIRA DE; GOMES, BRUNO COSTA; DEVOZ, PAULA PICOLI; DUARTE, NATHALIA DE ASSIS AGUILAR; RIBEIRO, DIEGO LUIS; ARAUJO, ADRIANA LADEIRA DE; BATISTA, BRUNO LEMOS; ANTUNES, LUSANIA MARIA GREGGI; BARBOSA, JR., FERNANDO; RODRIGUES, ANTONIO SEBASTIAO; et al. Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb). FRONTIERS IN GENETICS, v. 12, FEB 17 2021. (18/24643-1, 16/24520-1)