Levels of distillers grains in feedlot diets: fatty acid profile of L. dorsi muscle and gene expression of genes involved in lipid metabolism

• Grant number: 17/21784-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2017
• Effective date (End): November 30, 2018
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Otávio Rodrigues Machado Neto
• Grantee: João Victor Rosa Ishikawa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

As a result of increase in acquisition costs for the purchase of foods commonly used in diets for production animals, lower cost food alternatives have often been studied. An option that has recently been highlighted are distillery grain with soluble dry or wet (DDGS and WDGS), because it has arise as an option in Brazil as a consequence of the beginning of ethanol production from maize. In view of the above, the objectives of the present project will be to evaluate the effects of inclusion of distillery grains in diets with ground corn, on the profile of fatty acids and expression of genes related to lipid metabolism. Will be used 100 male bovines, F1 Angus-Nelore, with initial weight average of 370 kg. The animals will be randomly allocated to treatments with different levels of WDG (0; 15%, 30% and 45% of dietary DM) in diets containing 88.7% concentrate and 11.3% bulky. The animals will be slaughtered using the brain concussion technique. The carcasses shall be identified, washed, divided into two halves and taken to the cold room for approximately 24 hours at 1 ° C. At boning, samples of the L. dorsi muscle will be collected, for the analysis of fatty acid profile. The samples to be subjected to gene expression analysis will be collected immediately after slaughter and kept at -80 ° C. (AU)