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Phenotypic and functional analysis of Th1 polarizing monocyte derived dendritic cells

Grant number: 17/17585-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2017
Effective date (End): November 30, 2019
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Telma Miyuki Oshiro Sumida
Grantee:Marina Mazzilli Ortega
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Immunotherapy based on monocyte derived dendritic cells (MoDCs) constitute a promises strategy for treatment of HIV infected patients. Protocols for obtention of mature MoDCs, properly capable to present antigen for specific T lymphocytes in general use IL-4 and GM-CSF cytokines as mediators of cell differentation, that originate DC's immature with myeloid characteristics. Stimulus with pro-inflammatory cytokines activate DC's, making them fit to exhibit antigen and activate a specific immune response. More recently some authors have proposed activation of DCs with type I and II IFN and TLR agonists to improve DCs maturation, called aDC1. aDC1 exhibits ability to produce high levels of IL-12p70, carrying to a potent response Th1, with CTL's stimulus. Considering our interest studying aDC1 for application on anti-HIV immunotherapy protocol and an ongoing project that study aDC-1 derived from infected individuals, the present study aims to investigate phenotypic and functional profile of aDC1 derived from monocytes of healthy donors. First will be performed a comparison between polarizing aDC1 and non-polarizing DCs and results obtained will be also used to complement ongoing study, using samples of infected individuals, in order to evaluate possible differences concerning profile of response between infected and non infected individuals. (AU)