Proteolytic processing analysis in the secretome of melanoma tumor cells

• Grant number: 17/24560-6
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Effective date (Start): January 31, 2018
• Effective date (End): March 30, 2018
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: André Zelanis Palitot Pereira
• Grantee: Isabella Fukushima
• Supervisor: Bruno José Fernandes Oliveira Manadas
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil
• Research place: Universidade de Coimbra (UC), Portugal
• Associated to the scholarship: 17/07897-7 - Proteolytic processing analysis in the secretome of melanoma tumor cells, BP.IC

Abstract

The search for biomarkers has been addressed by several investigators in the field of cancer biology, however, given the multitude of etiologic factors associated to oncogenesis, a number of challenges still need to be overcome. The imbalance of cellular homeostasis during oncogenesis has a marked effect on the repertoire of the proteins secreted by malignant cells (cellular secretome), as well as, upon the physiological processing events that occurs during or after protein synthesis. The development and improvement of high throughput analytical techniques provide main evidences regarding the complexity in ordinary physiological mechanisms of eukaryotic cells, as well as pathological processes such as cancer. In this context, proteolytic processing is responsible for the generation of new protein N-termini and comprises an essentially irreversible cell signaling process, affecting a number of biological pathways with important implications in oncogenesis. Therefore, the main goal of this project is to identify the peptidome diversity derived from proteolytic processing events in the secretome of cultured tumor cells using Data Independent Acquisition approaches in mass spectrometry and correlating the results with biological pathways that might be eventually involved in the oncogenesis process. (AU)