Diabetes mellitus (DM) is the most prevalent endocrinopathy around the world. Recently, many studies have indicated a positive correlation between DM and thyroid dysfunctions. Indeed, studies in our laboratory have detected primary hypothyroidism in rats with alloxan-induced type 1 diabetes. The triiodothyronine (T3) treatment promoted enhancement of insulin sensitivity and reduced glycemia, alterations that were accompanied by reduction of pro-inflammatory cytokines, which are involved on peripheral insulin resistance in DM1 as well as in DM2. It is known that T3 acts by regulating genes expression at transcriptional as well as post transcriptional levels. It is also known that 60% of mRNAs are target of miRNAs which have been emerged as important markers and therapeutic targets of diseases as DM. In this context, investigate the function of T3 on regulation of expression of miRNAs in tissues that are essential for the maintenance of glycemia, could amplify our knowledge about the effects of this hormone on DM. Therefore, the aim of this study is identify the miRNAs expression profile in liver of DM1 rats and in mesenteric white adipose tissue of obese rats treated or not with T3, by real time polymerase chain reaction (qPCR) and in silico analysis. The miRNAs differentially expressed which exhibit potential therapeutic target of T3 in DM will be functionally validated by cell lineages. The results obtained will expand the field of knowledge in this area, leading to important therapeutic perspectives.
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