Triiodothyronine (T3) treatment effects on miRNA expression profile in type 1 diabetes mellitus and obesity: importance and therapeutic implications

• Grant number: 17/21875-6
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): December 01, 2017
• Effective date (End): March 31, 2020
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Maria Tereza Nunes
• Grantee: Ana Carolina Panveloski Costa Salomão
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/05629-4 - Genomic vs nongenomic actions of thyroid hormones: changes of paradigms, physiological implications and therapeutical perspectives, AP.TEM

Abstract

Diabetes mellitus (DM) is the most prevalent endocrinopathy around the world. Recently, many studies have indicated a positive correlation between DM and thyroid dysfunctions. Indeed, studies in our laboratory have detected primary hypothyroidism in rats with alloxan-induced type 1 diabetes. The triiodothyronine (T3) treatment promoted enhancement of insulin sensitivity and reduced glycemia, alterations that were accompanied by reduction of pro-inflammatory cytokines, which are involved on peripheral insulin resistance in DM1 as well as in DM2. It is known that T3 acts by regulating genes expression at transcriptional as well as post transcriptional levels. It is also known that 60% of mRNAs are target of miRNAs which have been emerged as important markers and therapeutic targets of diseases as DM. In this context, investigate the function of T3 on regulation of expression of miRNAs in tissues that are essential for the maintenance of glycemia, could amplify our knowledge about the effects of this hormone on DM. Therefore, the aim of this study is identify the miRNAs expression profile in liver of DM1 rats and in mesenteric white adipose tissue of obese rats treated or not with T3, by real time polymerase chain reaction (qPCR) and in silico analysis. The miRNAs differentially expressed which exhibit potential therapeutic target of T3 in DM will be functionally validated by cell lineages. The results obtained will expand the field of knowledge in this area, leading to important therapeutic perspectives.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PANVELOSKI-COSTA, ANA CAROLINA; TATAGIBA KUWABARA, WILSON MITSUO; MUNHOZ, ANA CLAUDIA; LUCENA, CAMILA FERRAZ; CURI, RUI; CARPINELLI, ANGELO RAFAEL; NUNES, MARIA TEREZA. The insulin resistance is reversed by exogenous 3,5,3 ` triiodothyronine in type 2 diabetic Goto-Kakizaki rats by an inflammatory-independent pathway. ENDOCRINE, v. 68, n. 2, SI, JAN 2020. (17/21875-6, 13/05629-4)