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Orexin: a gene methylation in women with obesity grade III submitted to hypocaloric diet

Grant number: 17/01101-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2017
Effective date (End): November 30, 2018
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal researcher:Carla Barbosa Nonino
Grantee:Bruna Aguiar Mansano
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Obesity, a disease that is part of the group of no communicable diseases (NCDs), is the excessive accumulation of body fat that has as a consequence damages to the health of individuals. Currently, this disease is a global public health problem, and both developed and developing countries show a high prevalence. The etiology of obesity is a multifactorial process involving environmental and genetic aspects. Interindividual particularities have often been attributed to genetic variants affecting hundreds of genes associated with energy metabolism, such as orexins. Several papers on the biological effects of obesity involving a genetics and metabolism have been discussed. In fact, there is increasing evidence that one of the mechanisms by which nutrients and bioactive compounds affect metabolic characteristics is epigenetics, such as the complex interactions between food components and DNA methylation. Objective: Characterize a difference in relation to the methylation of the orexin A gene (hypocrite 1) in women with grade III obesity and eutrophic women; To investigate the impact of a hypocaloric diet on the methylation of the orexin A gene in women with level III obesity. Methodology: Refers to a longitudinal prospective study in which 20 women aged 18-60 years will be elected, who will be separated into two groups: 1. Diet group: 10 women with grade III obesity (body mass index - BMI> 40 kg / m²) who will undergo nutritional intervention (hypocaloric diet) over 8 weeks and 2. Control group: 10 healthy women (BMI Between 18.5 and 24.9 kg / m²). Patients will be submitted to weight, height, BMI, body composition, waist circumference and food consumption (24 hour recall). In addition, peripheral blood will be collected for DNA extraction from the GE Health Care kit (Illustrate blood genomic Prep Mini Spin kit) with the purpose of analyzing the methylation of the Orexin A gene by the MS-HRM technique after the modification of DNA samples by sodium. Statistical analysis: The normality of the data will be evaluated by the Shapiro-Wilk test. The paired t test will be used to compare the results before and after the intervention. Significance analyzes will be performed in the Statistical Package for Social Science, considering p <0.05. (AU)

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