Characterization of MAPKs dependent-phosphoproteomes of Aspergillus fumigatus under stress conditions

• Grant number: 17/19288-5
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): February 01, 2018
• Effective date (End): January 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Gustavo Henrique Goldman
• Grantee: Eliciane Cevolani Mattos
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 16/07870-9 - The influence of mitogen activated protein kinases (MAPK) on the expression of genetic determinants important for Aspergillus fumigatus virulence, AP.TEM
• Associated scholarship(s): 18/18043-1 - Characterization of phospho-mimetic mutants of kinases in Aspergillus fumigatus by CRISPR-cas 9 gene edition, BE.EP.PD

Abstract

Aspergillus fumigatus is an opportunistic and allergenic pathogenic fungus, responsible for about 65% of fungal infections in humans and for several pathologies in immunocompromised individuals, including invasive aspergillosis (IA). A. fumagatus has four MAPKs described: MpkA (central regulator of the CWI signaling pathway and acting in response to oxidative stress); MpkB (not well characterized); MpkC and SakA (orthologs of S. cerevisiae- Hog1, a kinase involved with the control of osmolarity glycerol response (HOG) pathway). Current studies are interested in the understand of the role of MAPKs in the cellular signaling triggered by treatment with stress-inductor molecules, as sorbitol or congo-red and caspofungin. It was recently demonstrated the involvement of Hog1 orthologous: SakA and MpkC, in the response to different cellular stresses as osmotic and oxidative stress, and in the fungus adaptation to caspofungin and in the use of carbon sources respectively. In addition, MpkA has been related with the pathogen response to the caspofungin stress. The proteome and phosphoproteome analyzes are important strategies for the identification of signaling pathways modulated by phosphorylation during a stress suffered by organisms. In this way, the present project has as main objectives the analyze of the phosphoproteome of null mutants of MAPKs (MpkC and SakA and MpkA) during the respective stresses: treatment with sorbitol or congo-red and caspofungin, in order to identify new elements of cellular signaling mediated by MAPKs during the pathogen response to the environmental conditions

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANFIOLLI, ADRIANA OLIVEIRA; MATTOS, ELICIANE CEVOLANI; DE ASSIS, LEANDRO JOSE; SILVA, LILIAN PEREIRA; ULAS, MEVLUT; BROWN, NEIL ANDREW; SILVA-ROCHA, RAFAEL; BAYRAM, OZGUR; GOLDMAN, GUSTAVO H.. Aspergillus fumigatus High Osmolarity Glycerol Mitogen Activated Protein Kinases SakA and MpkC Physically Interact During Osmotic and Cell Wall Stresses. FRONTIERS IN MICROBIOLOGY, v. 10, MAY 7 2019. (16/07870-9, 17/19288-5, 14/00789-6)

DE CASTRO, PATRICIA ALVES; COLABARDINI, ANA CRISTINA; MANFIOLLI, ADRIANA OLIVEIRA; CHIARATTO, JESSICA; SILVA, LILIAN PEREIRA; MATTOS, ELICIANE CEVOLANI; PALMISANO, GIUSEPPE; ALMEIDA, FAUSTO; PERSINOTI, GABRIELA FELIX; ANNICK RIES, LAURE NICOLAS; et al. Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance. PLOS GENETICS, v. 15, n. 12, DEC 2019. (17/07536-4, 16/21392-2, 14/06863-3, 16/12948-7, 16/07870-9, 16/22062-6, 14/04783-2, 17/19288-5)

MATTOS, ELICIANE CEVOLANI; SILVA, LILIAN PEREIRA; VALERO, CLARA; DE CASTRO, PATRICIA ALVES; DOS REIS, THAILA FERNANDA; RIBEIRO, LILIANE F. C.; MARTEN, MARK R.; SILVA-ROCHA, RAFAEL; WESTMANN, CAUA; TOMICH DE PAULA DA SILVA, CARLOS HENRIQUE; et al. The Aspergillus fumigatus Phosphoproteome Reveals Roles of High-Osmolarity Glycerol Mitogen-Activated Protein Kinases in Promoting Cell Wall Damage and Caspofungin Tolerance. MBIO, v. 11, n. 1, JAN-FEB 2020. (18/18043-1, 16/07870-9, 17/19288-5)