Validation of the better intracellular cryoprotectant for boar semen cryopreservation in One Step method followed by comparation with Two Step method

• Grant number: 17/16987-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2017
• End date: October 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: André Furugen Cesar de Andrade
• Grantee: Matheus Saliba Monteiro
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The boar semen is still little used and this occurs not only because the process causes reduction on the fertility rate. The current method of cryopreservation of boar semen is the Two Steps. This method is expensive and requires a long time, equipment and labor, fact that contributes to reduce the utilization of this biotechnology. This experiment aims to create simpler and faster methods that improves the post-thawed semen using smaller amounts of equipment and labor during the cryopreservation. Thus, two experiments will be realized. Experiment 1: Aims verify which Cryoprotectant penetrant agent (CPA) is better: 5 % Acetamide; 5% Dimethylformamide; 1% Glycerol and 4% Dimethylformamide; 2% Glycerol and 2% Dimethylformamide. The second experiment aims to compare the One Step method with the methodology commonly used in the boar semen cryopreservation, which is the Two Step. For this will be compared the best cryoprotecant found in the experiment 1 with the Two Step method Westendorf et al., 1975 and adapted by Yeste et al., 2016. After thawing, samples will be evaluated by motility characteristics (CASA), head's morfometry (ASMA), plasma and acrosomal membrane integrity, mitochondrial membrane potential, fluidity of plasma membrane lipids and lipoperoxidation by flow cytometer. Data will be submitted to analysis of mixed models (PROC MIXED), using the SAS (2002). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MONTEIRO, MATHEUS SALIBA; TORRES, MARIANA ANDRADE; PASSARELLI, MARINA DA SILVA; MARTINS, MATHEUS PASSINI; RAVAGNANI, GISELE MOURO; PAPA, FREDERICO OZANAM; ALVARENGA, MARCO ANTONIO; DELL'AQUA JUNIOR, JOSE ANTOMO; YASUI, GEORGE SHIGUEKI; MARTINS, SIMONE MARIA MASSAMI KITAMURA; et al. Impact of cryopreservation protocols (one- and two-step) on boar semen quality at 5 degrees C and post-thawing. Animal Reproduction Science, v. 247, p. 9-pg., 2022-10-20. (17/16987-0, 16/24690-4, 17/10821-2)