Preparation of chromosome translocations probes of Mazama gouazoubira species by microdissection

• Grant number: 17/21333-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2017
• Effective date (End): December 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: José Maurício Barbanti Duarte
• Grantee: Agda Maria Bernegossi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 18/06780-1 - Generation of translocated chromosomes probes of the Mazama gouazoubira species by microdissection, BE.EP.IC

Abstract

Mazama gouazoubira (2n = 70; FN = 70) is a Brazilian neotropical deer popularly known as brown brocket deer. Is characterized by having retained the ancestral karyotype of the Cervidae family and by the presence of Robertsonian translocations. A recent study, which used G-banding technique, identified seven different types of translocations (X; 16; X; 21; 7; 21; 8; 21; 4; 16; 20; 26; 14; 16), involving nine different chromosomes in the Brazilian population of M. gouazoubira. However, confirmation of sharing the same type of translocation in different animals through fluorescent in situ hybridization (FISH) was unable due to the difficulties in molecular cytogenetic techniques. Given the above, this study aims to use advanced techniques in the detection and confirmation of chromosomal rearrangements occurred in the brown brocket deer. In this regard, this study aims to prepare probes by microdissection of translocated chromosomes of individuals that presented different types of translocation. In order to confirm whether the same type of translocation is shared by different animals, the probes will be used in the FISH technique. Furthermore, these probes will be hybridized in individuals without Robertsonian translocations, in order to establish of chromosomal markers for the M. gouazoubira species, since all their chromosomes have similar morphology and size, making it difficult to individualize and differentiate each chromosomal pair. It is expected that the results found at the end of this study contribute as a reference base for later studies, making it possible to elucidate doubts regarding the karyotype evolution of the Cervidae family. (AU)