Inhibitor drugs of the renin-angiotensin system: antimicrobial action against Candida albicans

• Grant number: 17/19294-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2018
• Effective date (End): January 31, 2019
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Vanessa Soares Lara
• Grantee: Tatiane Ponteado Ferrari
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated research grant: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach, AP.TEM

Abstract

This study intends to evaluate in vitro if the drugs Alisquirene, Losartan and Punica granatum, that interfere in the renin-angiotensin system, also present antimicrobial action against biofilms of Candida albicans, and alter the viability of human gingival fibroblasts (HGF). Antimicrobial analysis will be performed by means of XTT reduction reduction assay, allowing the determination of the level of metabolic activity of viable cells and, finally, the minimum inhibitory concentration (MIC). The cytotoxicity of the different drugs on HGF will be evaluated by means of fluorescein diacetate and Alarmar Blue. With this proposal, we intend to make possible in the future the idea of associating antifungal drugs with the resilient materials used on the inner surface of removable dental prostheses, since it functions as a reservoir of microorganisms, mainly Candida albicans, which is strongly involved with the pathogenesis of Denture Stomatitis. (AU)