EFFECT OF PHOTOBIOMODULATION IN THE MESENQUIMIAL TRUNK CELLS OF ADIPOSE TISSUE IN 2D AND 3D CULTURE

• Grant number: 17/23167-9
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): February 20, 2018
• Effective date (End): September 01, 2018
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Nivaldo Antonio Parizotto
• Grantee: Ana Laura Martins de Andrade
• Supervisor: Alexandra P. Marques
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Research place: Universidade do Minho (UMinho), Portugal
• Associated to the scholarship: 15/17648-9 - EFFECTS OF LOW LEVEL LASER THERAPY (660nm) ASSOCIATED WITH MESENCHYMAL STEM CELLS IN THIRD-DEGREE BURNS: STUDY "IN VITRO" E "IN VIVO"., BP.DR

Abstract

The use of mesenchymal stem cells (CTMs) in tissue engineering has been extensively investigated by researchers. It is known that the greater the proliferation of this cellular group the greater the regenerative and cicatricial capacity of the tissues where they reside. In this context, photobiomodulation therapy (PBM) is presented in the literature as an efficient technique in the proliferation of several cell types, but the parameterization and the mode of action of this technique are not yet elucidated, making its investigation necessary. Studies with 3D cell culture have been widely explored, due to the three-dimensionality offered, facilitating the understanding of cellular reactions in an environment with characteristics closer to "in vivo". The objective of this study was to evaluate the action of PBM, with different energies, on adipose tissue MTCs in 2D and 3D cell culture with gellan gellan hydrogel and hyaluronic acid (GG-HA). Adenoviral mesenchymal stem cells (hASCs) isolated for 2D culture and associated with GG-HA will be used for the construction of the culture in 3D. There will be eight experimental groups, a group with hASCs and simulation of PBM, a group with hASCs + GG-HÁ also with simulation of PBM and the other six groups that will receive the PBM irradiation, in the times of 24, 48 and 72 hours, with a laser of 660nm, power of 40mW and energies of 0.56; 1.96; 5.04J. Microstructure analysis will be performed through DAPI labeling and cytotoxicity and proliferation analyzes. In addition, an evaluation of the biochemical characteristics in the secretoma of hASCs will be performed.