Molecular and sorological detection of Anaplasma marginale in beef cattle from Pantanal sul matogrossense

• Grant number: 17/23327-6
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2018
• Effective date (End): January 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal researcher: Marcos Rogério André
• Grantee: Bruna de Carvalho Le Sénéchal Horta
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Anaplasma marginale is an intra-erythrocytic bacterium that can be transmitted via placenta, biologically by ticks and mechanically by blood-sucking insects or fomites contaminated with blood. Infection with this agent results in additional costs for cattle rearing for the milk and meat industries, characterized by severe anemia that causes decreased weight gain and milk production, and frequently death. In Brazil, its prevalence ranges from 12.3% to 100%, and this pathogen can establish persistent infection in cattle. The present study aimed to investigate the occurrence of Anaplasma marginale in naturally infected beef herds in the Pantanal of Mato Grosso do Sul, using serological and molecular techniques. Serum and blood samples will be submitted to Indirect Enzyme-Linked Immunosorbent Assay [iELISA]) and Real-time Quantitative PCR [qPCR]), respectively. The data obtained here will contribute to the knowledge of the occurrence of Anaplasma marginale in Pantanal. In addition, considering that the Pantanal Sul Matogrossense is responsible for beef cattle production, the impacts on cattle breeding in this region are of great economic importance for the country. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDRE, MARCOS ROGERIO; CALCHI, ANA CLAUDIA; HERRERA, HEITOR MIRAGLIA; DE SOUZA ZANATTO, DIEGO CARLOS; LE SENECHAL HORTA, BRUNA DE CARVALHO; TASSO, JULIA BANHARELI; DE SOUZA RAMOS, INALDA ANGELICA; CALIFRE DE MELLO, VICTORIA VALENTE; MACHADO, ROSANGELA ZACARIAS. The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 21, JUL 2020. Web of Science Citations: 1.