MicroRNAs in stool and platelets biology from gastroenterology cancer patient samples that correlate with ACP1 protein tyrosine phosphatase: colorectal cancer screening

• Grant number: 18/00736-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): February 25, 2018
• Effective date (End): February 24, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Carmen Veríssima Ferreira
• Grantee: Alessandra Valéria de Sousa Faria
• Supervisor: Maikel Peppelenbosch
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: Erasmus University Rotterdam (EUR), Netherlands
• Associated to the scholarship: 17/08119-8 - Hematogenous tumor metastasis in colon rectal cancer cells: influence of LMWPTP and 3-bromopyruvate, BP.DR

Abstract

The success of cancer prevention and treatment involves efficiency diagnosis and therapy. Over the past years, the Prof. Ferreira-Halder and Prof. Peppelenbosch research groups have investigated cancer cells metabolism to clarify resistance and aggressiveness processes. It was found a correlation between resistance/aggressiveness in leukemia, prostate and colorectal cancer (CRC) and high expression of Low Weight Molecular Protein Tyrosine Phosphatase (LMWPTP). This proposal will address two important points in cancer field: validation of a newbiomarker (LMWPTP and/or molecules related to LMWPTP) and biological characterization of a small molecule (3-bromopyruvate) that has a potential to re-educate platelets and consequently, inhibits the first step ofhematogenous dissemination of CRC cells. Therefore, the aims will cover: 1) for diagnosis: identify LMWPTP ormiRNAs modulated by this enzyme in fecal samples from patients and used it as a biomarker. Since, the mostused diagnostic testing for colorectal cancer has low sensitivity (fecal occult blood testing), or it is expensive andinvasiveness (colonoscopy), new biomarker that overcomes these problems is urgently needed; and 2) fordecreasing metastasis efficiency: 3-bromopyruvate is able to decrease the interaction between CRC cellsplatelets.This finding indicates that this small molecule has a greater potential to block the metastasis. At this stage, we will scrutinize the molecular mechanism by which it occurs. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FARIA, ALESSANDRA V. S.; ANDRADE, SHEILA S.; PEPPELENBOSCH, MAIKEL P.; FERREIRA-HALDER, CARMEN V.; FUHLER, GWENNY M.. Platelets in aging and cancer-{''}double-edged sword{''}. CANCER AND METASTASIS REVIEWS, v. 39, n. 4, SI, SEP 2020. (17/08119-8, 17/26317-1, 16/14459-3, 15/20412-7, 18/00736-0)

FARIA, ALESSANDRA V. DE S.; AKYALA, ADAMU ISHAKU; PARIKH, KAUSHAL; BRUEGGEMANN, LOIS W.; SPEK, C. ARNOLD; CAO, WANLU; BRUNO, MARCO J.; BIJLSMA, MAARTEN F.; FUHLER, GWENNY M.; PEPPELENBOSCH, MAIKEL P.. Smoothened-dependent and -independent pathways in mammalian noncanonical Hedgehog signaling. Journal of Biological Chemistry, v. 294, n. 25, p. 9787-9798, JUN 21 2019. (18/00736-0)

FARIA, ALESSANDRA V. S.; ANDRADE, SHEILA S.; PEPPELENBOSCH, MAIKEL P.; FERREIRA-HALDER, V, CARMEN; FUHLER, GWENNY M.. The role of phospho-tyrosine signaling in platelet biology and hemostasis. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1868, n. 3, MAR 2021. (15/20412-7, 17/26317-1, 17/08119-8, 16/14459-3, 18/00736-0)

LIESHOUT, RUBY; FARIA, ALESSANDRA V. S.; PEPPELENBOSCH, MAIKEL P.; VAN DER LAAN, LUC J. W.; VERSTEGEN, MONIQUE M. A.; FUHLER, GWENNY M.. Kinome profiling of cholangiocarcinoma organoids reveals potential druggable targets that hold promise for treatment stratification. Molecular Medicine, v. 28, n. 1, p. 15-pg., 2022-12-01. (18/00736-0, 17/08119-8)

FARIA, ALESSANDRA V. S.; ANDRADE, SHEILA S.; PEPPELENBOSCH, MAIKEL P.; FERREIRA-HALDER, CARMEN V.; FUHLER, GWENNY M.. Platelets in aging and cancer-"double-edged sword". CANCER AND METASTASIS REVIEWS, v. 39, n. 4, p. 17-pg., 2020-09-01. (16/14459-3, 17/08119-8, 18/00736-0, 17/26317-1, 15/20412-7)

FARIA, ALESSANDRA V. S.; YU, BINGTING; MOMMERSTEEG, MICHIEL; DE SOUZA-OLIVEIRA, PATRICIA F.; ANDRADE, SHEILA S.; SPAANDER, MANON C. W.; DE MAAT, MONIEK P. M.; PEPPELENBOSCH, MAIKEL P.; FERREIRA-HALDER, V, CARMEN; FUHLER, GWENNY M.. Platelet-dependent signaling and Low Molecular Weight Protein Tyrosine Phosphatase expression promote aggressive phenotypic changes in gastrointestinal cancer cells. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1868, n. 1, JAN 1 2022. (17/26317-1, 18/00736-0, 16/14459-3, 17/08119-8, 15/20412-7)

FARIA, ALESSANDRA V. S.; ANDRADE, SHEILA S.; REIJM, AGNES N.; SPAANDER, MANON C. W.; DE MAAT, MONIEK P. M.; PEPPELENBOSCH, MAIKEL P.; FERREIRA-HALDER, V, CARMEN; FUHLER, GWENNY M.. Targeting Tyrosine Phosphatases by 3-Bromopyruvate Overcomes Hyperactivation of Platelets from Gastrointestinal Cancer Patients. JOURNAL OF CLINICAL MEDICINE, v. 8, n. 7, JUL 2019. (17/08119-8, 18/00736-0, 15/20412-7)