Zika virus in postpartum women and newborns: seroepidemiology and molecular characterization

• Grant number: 17/24769-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: January 01, 2018
• End date: November 30, 2022
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Edison Luiz Durigon
• Grantee: Rafael Rahal Guaragna Machado
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV), AP.TEM
• Associated scholarship(s): 19/27803-2 - Immune-mediated mayaro virus protection from wild-type chikungunya virus infection, BE.EP.DD

Abstract

The Zika Virus (ZIKV) is an arbovirus belonging to the family Flaviviridae, genus Flavivirus. Arboviruses are viruses characterized by being kept in the wild in enzootic cycles, involving hematophagous arthropod vectors and may participate in an urban cycle in which humans are the main hosts. In 2015, there was an epidemiological outbreak of ZIKV in Brazil, which is considered by the World Health Organization as an emergency public health emergency pathogen of international concern. In addition to the mode of vector transmission, by species of the genus Aedes, other non-vector transmission media have already been reported, such as sexual, vertical, transfusional, saliva and urine transmission. About 80% of cases of ZIKV infection are asymptomatic and, when present, clinical manifestations are very similar to other arbovirus infections, making the diagnosis of this disease a major challenge. Currently, even though several clinical complications are known, following the ZIKV infection, few studies aimed to investigate the serological profile of these patients. Therefore, this project aims to conduct a prospective/descriptive/epidemiological study, aiming to investigate the frequency of ZIKV infection in puerperae and neonates, determining their serological profile and the molecular characterization of the Zika virus. This study is based on the importance of understanding the full scope of the effect of Zika virus in pregnancy, since it has not been elucidated yet, besides the need for epidemiological data that can help guide the medical and public health responses to this arbovirose. (AU)

Scientific publications

(19)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

OHKI, CRISTINE MARIE YDE; BENAZZATO, CECILIA; VAN DER LINDEN, VANESSA; FRANCA, JULIA, V; TOLEDO, CARMEN M.; MACHADO, RAFAEL RAHAL GUARAGNA; ARAUJO, DANIELLE BASTOS; OLIVEIRA, DANIELLE BRUNA LEAL; NERIS, ROMULO S.; ASSUNCAO-MIRANDA, IRANAIA; et al. Zika virus infection impairs synaptogenesis, induces neuroinflammation, and could be an environmental risk factor for autism spectrum disorder outcome. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1870, n. 5, p. 13-pg., 2024-04-17. (13/08844-3, 18/16748-8, 17/24769-2, 11/20683-0, 16/02978-6, 16/20045-7, 17/27131-9)

MACHADO, RAFAEL RAHAL GUARAGNA; CANDIDO, ERIKA DONIZETTI; AGUIAR, ANDRESSA SIMOES; CHALUP, VANESSA NASCIMENTO; SANCHES, PATRICIA ROMAO; DORLASS, ERICK GUSTAVO; AMGARTEN, DEYVID EMANUEL; PINHO, JOAO RENATO REBELLO; DURIGON, EDISON LUIZ; OLIVEIRA, DANIELLE BRUNA LEAL. Immune Evasion of SARS-CoV-2 Omicron Subvariants XBB.1.5, XBB.1.16 and EG.5.1 in a Cohort of Older Adults after ChAdOx1-S Vaccination and BA.4/5 Bivalent Booster. VACCINES, v. 12, n. 2, p. 13-pg., 2024-02-01. (20/06409-1, 17/24769-2)

DE SOUZA, ALEX JUNIOR SOUZA; DE SOUZA, ANTONIO FRANCISCO; ZIMPEL, CRISTINA KRAEMER; AYUPE, MARINA CACADOR; DE ARAUJO, MARCELO VALDEMIR; MACHADO, RAFAEL RAHAL GUARAGNA; SALLES, ERIKA; SALGADO, CAIO LOUREIRO; TAVARES, MARIANA SILVA; SILVA-PEREIRA, TAIANA TAINA; et al. Hepatic endotheliitis in Golden Syrian hamsters (Mesocricetus auratus) experimentally infected with SARS-CoV-2. Revista do Instituto de Medicina Tropical de São Paulo, v. 66, p. 6-pg., 2024-01-01. (21/02736-0, 21/06881-5, 20/06409-1, 23/00532-4, 17/24769-2, 20/07251-2, 19/10896-8, 20/09149-0, 16/20045-7)

VENCESLAU-CARVALHO, ALEXIA ADRIANNE; FAVARO, MARIANNA TEIXEIRA DE PINHO; PEREIRA, LENNON RAMOS; RODRIGUES-JESUS, MONICA JOSIANE; PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; ALVES, RUBENS PRINCE DOS SANTOS; CASTRO-AMARANTE, MARIA FERNANDA; RODRIGUES, KARINE BITENCOURT; DA SILVA, JAMILE RAMOS; et al. Nano-multilamellar lipid vesicles loaded with a recombinant form of the chikungunya virus E2 protein improve the induction of virus-neutralizing antibodies. Nanomedicine-Nanotechnology Biology and Medicine, v. 37, OCT 2021. (17/23281-6, 16/19145-7, 16/08204-2, 17/24769-2, 16/20045-7, 16/23560-0)

DANIELLE BASTOS ARAUJO; RAFAEL RAHAL GUARAGNA MACHADO; DEYVID EMANUEL AMGARTEN; FERNANDA DE MELLO MALTA; GABRIEL GUARANY DE ARAUJO; CAIRO OLIVEIRA MONTEIRO; ERIKA DONIZETTI CANDIDO; CAMILA PEREIRA SOARES; FERNANDO GATTI DE MENEZES; ANA CAROLINA CORNACHIONI PIRES; et al. SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. Memórias do Instituto Oswaldo Cruz, v. 115, 2020-10-23. (16/20045-7, 18/23680-0, 20/06409-1, 20/04680-0, 17/24769-2, 18/21076-9, 17/25123-9)

MENDRONE-JUNIOR, ALFREDO; DINARDO, CARLA LUANA; FERREIRA, SUZETE CLEUZA; NISHYA, ANNA; SALLES, NANCI ALVES; DE ALMEIDA NETO, CESAR; HAMASAKI, DEBORA TOSHEI; FACINCANI, TILA; DE OLIVEIRA ALVES, LUCAS BASSOLLI; MACHADO, RAFAEL RAHAL GUARAGNA; et al. Correlation between SARS-COV-2 antibody screening by immunoassay and neutralizing antibody testing. Transfusion, v. 61, n. 4, p. 1181-1190, APR 2021. (16/20045-7, 20/06409-1, 17/24769-2)

DE PAULIS, MILENA; OLIVEIRA, DANIELLE BRUNA LEAL; VIEIRA, RODOLFO P.; PINTO, ISABELLA COUTINHO; MACHADO, RAFAEL RAHAL GUARAGNA; CAVALCANTI, MARIANA PAUFERRO; SOARES, CAMILA PEREIRA; DE ARAUJO, ANANDA MEDEIROS PEREIRA; ARAUJO, DANIELLE BASTOS; BACHI, ANDRE LUIS LACERDA; et al. Multisystem Inflammatory Syndrome Associated With COVID-19 With Neurologic Manifestations in a Child: A Brief Report. PEDIATRIC INFECTIOUS DISEASE JOURNAL, v. 39, n. 10, p. 4-pg., 2020-10-01. (16/04845-3, 18/23680-0, 20/06409-1, 12/15165-2, 17/24769-2)

WENDEL, SILVANO; KUTNER, JOSE MAURO; MACHADO, RAFAEL; FONTAO-WENDEL, RITA; BUB, CAROLINA; FACHINI, ROBERTA; YOKOYAMA, ANA; CANDELARIA, GABRIELA; SAKASHITA, ARACI; ACHKAR, RUTH; et al. Screening forSARS-CoV-2 antibodies in convalescent plasma in Brazil: Preliminary lessons from a voluntary convalescent donor program. Transfusion, v. 60, n. 12, p. 2938-2951, DEC 2020. (18/23680-0, 17/24769-2, 16/20045-7, 20/06409-1)

MACHADO, RAFAEL R. G.; GLASER, TALITA; ARAUJO, DANIELLE B.; PETIZ, LYVIA LINTZMAIER; OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; LEAL, ALESSANDRA L.; PINHO, JOAO RENATO R.; FERREIRA, LUIS C. S.; ULRICH, HENNING; et al. Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 Replication by Hypertonic Saline Solution in Lung and Kidney Epithelial Cells. ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE, v. 4, n. 5, p. 1514-1527, OCT 8 2021. (20/06409-1, 19/00195-2, 18/07366-4, 17/24769-2, 16/20045-7, 20/04680-0)

GOMES, VINICIUS DE MORAIS; SANTOS, DEIVID MARTINS; MACEDO-DA-SILVA, JANAINA; LAZARI, LUCAS C.; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS, ANCELY FERREIRA; ARAUJO, DANIELLE BASTOS; COUTINHO, JOAO VITOR PACCINI; ARINI, GABRIEL SANTOS; ANGELI, CLAUDIA B.; et al. P.1 and P.2 SARS-CoV-2 Brazilian variants activate the unfolded protein response with a time and pathway specificity. JOURNAL OF PROTEOMICS, v. 315, p. 17-pg., 2025-02-21. (20/06747-4, 16/04676-7, 15/26722-8, 19/09517-2, 22/13150-0, 21/14179-9, 20/06409-1, 18/15549-1, 18/07142-9, 18/18257-1, 17/24769-2, 16/20045-7, 20/04923-0, 21/00140-3)

MACHADO, RAFAEL RAHAL GUARAGNA; PERES, MARCELLA SANCHES; PEREIRA, SAMUEL SANTOS; MELLO, RALYRIA; ARAUJO, DANIELLE BASTOS; PEREIRA, CAMILA SOARES; PASTORINO, BORIS; OLIVEIRA, DANIELLE BRUNA LEAL; FALEIROS, MICHELE DA SILVA JORDAN; FRANSCISCO, ROSSANA PULCINELI VIEIRA; et al. Seroprevalence and Placental Transfer of Zika and Dengue Virus Antibodies in Postpartum Women in Southeast Brazil. Journal of Medical Virology, v. 97, n. 5, p. 15-pg., 2025-05-01. (16/20045-7, 19/16975-7, 20/06409-1, 17/24769-2)

PRUDENCIO, CARLOS ROBERTO; DA COSTA, VIVALDO GOMES; ROCHA, LETICIA BARBOZA; DA COSTA, HERNAN HERMES MONTEIRO; ORTS, DIEGO JOSE BELATO; SANTOS, FELIPE ROCHA DA SILVA; RAHAL, PAULA; LINO, NIKOLAS ALEXANDER BORSATO; DA CONCEICAO, PAMELA JOYCE PREVIDELLI; BITTAR, CINTIA; et al. Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests. Viruses-Basel, v. 15, n. 3, p. 20-pg., 2023-03-01. (16/20045-7, 16/16188-7, 17/50333-7, 16/23560-0, 15/17178-2, 16/05570-8, 17/24769-2)

ANDREATA-SANTOS, ROBERT; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; SOARES, CAMILA PEREIRA; RODRIGUES, KARINE BITENCOURT; SILVA, MARIANGELA OLIVEIRA; FAVARO, MARIANNA TEIXEIRA DE PINHO; RODRIGUES-JESUS, MONICA JOSIANE; YAMAMOTO, MARCIO MASSAO; et al. Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the Sao Paulo University Hospital, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 8-pg., 2022-06-02. (20/08943-5, 16/20045-7, 21/05661-1, 14/50890-5, 16/23560-0, 15/02352-7, 20/06409-1, 17/24769-2, 16/14344-1, 18/23680-0, 20/10700-3, 18/07629-5, 18/07142-9)

WENDEL, SILVANO; FONTAO-WENDEL, RITA; FACHINI, ROBERTA; CANDELARIA, GABRIELA; SCURACCHIO, PATRICIA; ACHKAR, RUTH; BRITO, MAYRA; REIS, LUIZ FERNANDO; CAMARGO, ANAMARIA; AMANO, MARIANE; et al. A longitudinal study of convalescent plasma (CCP) donors and correlation of ABO group, initial neutralizing antibodies (nAb), and body mass index (BMI) with nAb and anti-nucleocapsid (NP) SARS-CoV-2 antibody kinetics: Proposals for better quality of CCP collections. Transfusion, v. 61, n. 5, FEB 2021. (18/23680-0, 17/24769-2, 16/20045-7, 20/06409-1)

LOPES, ALESSANDRA G. D.; CELESTINO, CAMILA S. H.; BARROS, TIAGO T. A.; FEVEREIRO, ALINE G.; GEJER, DEBORA H.; OLIVEIRA, FERNANDO M. F.; BRASIL, JAMILE M.; BOSSOLAN, ROSELY M.; PINTO, GABRIELA C. C.; SANTOS, ANA C. E. Z.; et al. Case Report: A Severe SARS-CoV-2 Infection in a Teenager With Angelman Syndrome. FRONTIERS IN MEDICINE, v. 8, MAR 12 2021. (17/24769-2, 20/06409-1)

DE FREITAS DUTRA, VALERIA; BONET-BUB, CAROLINA; YOKOYAMA, ANA PAULA H.; ACHKAR, RUTH; MACHADO, RAFAEL RAHAL GUARAGNA; ASSUNCAO, MURILO; CANDELARIA, GABRIELA; SOARES, CAMILA PEREIRA; FACHINI, ROBERTA MARIA; FONTAO-WENDEL, RITA; et al. Anti-A and SARS-CoV-2: an intriguing association. Vox Sanguinis, v. 116, n. 5, MAR 2021. (18/23680-0, 17/24769-2, 16/20045-7, 20/06409-1)

DORLASS, ERICK GUSTAVO; MONTEIRO, CAIRO OLIVEIRA; VIANA, AMANDA OLIVEIRA; SOARES, CAMILA PEREIRA; GUARAGNA MACHADO, RAFAEL RAHAL; THOMAZELLI, LUCIANO MATSUMIYA; ARAUJO, DANIELLE BASTOS; LEAL, FABYANO BRUNO; CANDIDO, ERIKA DONIZETTE; TELEZYNSKI, BRUNA LAROTONDA; et al. Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR. Brazilian Journal of Microbiology, v. 51, n. 3, AUG 2020. (17/25123-9, 16/20045-7, 18/23680-0, 17/24769-2)

WENDEL, SILVANO; FACHINI, ROBERTA; FONTAO-WENDEL, RITA C. L.; MELLO, RALYRIA; VELASQUEZ, CARLOS VINICIUS; GUARAGNA MACHADO, RAFAEL RAHAL; BRITO, MAYRA ALTOBELLI; AMARAL, MARCELO; SOARES, CAMILA PEREIRA; ACHKAR, RUTH; et al. urrogate test performance for SARS-CoV-2 neutralizing antibodies (nAbs) for convalescent plasma (CCP): How useful could they be. Transfusion, v. 61, n. 12, OCT 2021. (17/24769-2, 20/06409-1, 16/20045-7, 18/23680-0)

SABINO, CAETANO P.; SELLERA, FABIO P.; SALES-MEDINA, DOUGLAS F.; GUARAGNA MACHADO, RAFAEL RAHAL; DURIGON, EDISON LUIZ; FREITAS-JUNIOR, LUCIO H.; RIBEIRO, MARTHA S.. UV-C (254 nm) lethal doses for SARS-CoV-2. Photodiagnosis and Photodynamic Therapy, v. 32, DEC 2020. (17/24769-2, 17/22406-0, 16/20045-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MACHADO, Rafael Rahal Guaragna. Seroprevalence of Zika (ZIKV) and Dengue (DENV) virus infection in parturients and transplacental transfer of anti-ZIKV and anti-DENV antibodies in newborns in Southeast, Brazil. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.