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Evaluation of the low-frequency ultrasound potential for the sonodynamic therapy of skin cancer

Grant number: 17/17442-7
Support type:Scholarships in Brazil - Master
Effective date (Start): January 01, 2018
Effective date (End): August 31, 2019
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Renata Fonseca Vianna Lopez
Grantee:Yugo Araújo Martins
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue, AP.TEM

Abstract

Sonodynamic therapy (SDT) is a therapeutic modality that has been studied for the non-invasive treatment of aggressive skin cancer such as melanoma. SDT involves the application of ultrasound on the tumor for the activation of sonosensitizing agent and the production of reactive oxygen species (ROS), which mediate the death of cancer cells. ROS generation is related to both the phenomenon of acoustic cavitation and the ultrasound frequency. Few studies have reported the effect of low-frequency ultrasound (LFU) on SDT, although LFU has extensively been studied in transdermal drug delivery. Therefore, LFU can be combined with SDT to facilitate skin penetration and activation of sonosensitizers. This work aims to investigate the influence of LFU on the SDT of melanoma associated or not with a sonosensitizer agent, chlorine-e6 (Ce6), topically administered, by utilizing both free and micelle form of Ce6. Cytotoxicity and in vitro production of ROS studies will be performed on murine melanoma cells under various conditions of LFU, Ce6 and micellar system application. In vitro skin penetration studies of Ce6 will be performed to investigate the influence of the micellar system and LFU on Ce6 penetration in skin. (AU)