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Transcriptional characterization of Pmch-expressing cells in the hypothalamus of lactating female mice (Mus musculus)

Grant number: 18/04659-0
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): June 11, 2018
Effective date (End): February 28, 2019
Field of knowledge:Biological Sciences - Morphology
Principal Investigator:Jackson Cioni Bittencourt
Grantee:Jozélia Gomes Pacheco Ferreira
Supervisor abroad: Alexander Jackson
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Connecticut (UCONN), United States  
Associated to the scholarship:15/05990-4 - The melanin-concentrating hormone [MCH] peptidergic system: neurogenesis and lactation, BP.PD

Abstract

Introduction: The melanin-concentrating hormone (MCH) is a neuropeptide associated with a lot of physiological actions located mainly in the lateral hypothalamic area (LHA). During lactation is possible to detect the MCH expressing cells (Pmch+) in the medial preoptic area (MPOA), a crucial region for maternal behavior expression. Whether the preoptic MCH neurons are similar to those located in the tuberal hypothalamus is unclear. As the preoptic MCH-immunoreactive cells are believed to act on a specific role, lactation, we expect to find at least some differences between tuberal and preoptic neurons. Aim: Draw the phenotypic profile of the Pmch+ cells of the preoptic hypothalamus of lactating female mice and compare it with that obtained from Pmch+ cells of the LHA. Materials and Methods: Genetically modified Pmch-EYFP+ female mice will be euthanized on the 19th day after delivery of their offspring through decapitation. Cells expressing the Pmch gene will be isolate using the Fluorescence Activated Cell Sorting (FACS) method and the phenotypic profile of the MPOA and LHA Pmch+ cells will be compared. (AU)