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Influence of different endodontic protocols on the dentin structure and in the adhesive dentin-sealer interface

Grant number: 18/03554-0
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 15, 2018
Effective date (End): October 14, 2019
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marco Antonio Hungaro Duarte
Grantee:Rafaela Fernandes Zancan
Supervisor abroad: Josette Camilleri
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Local de pesquisa : University of Birmingham, England  
Associated to the scholarship:16/25133-1 - Enterococcus faecalis: development of a final irrigant to destroy it, survival analysis of it after endodontic treatment and its ability to stimulate inflammatory cytokines after stress, BP.DR


Solutions used in endodontic treatment can alter root canal surface properties and composition, interfering with the adhesion of sealers. Free spaces along the obturation allows infiltration of microorganisms from the oral environment to apical region, leading to endodontic failure. This study will be divided into two stages: In stage 1 in Brazil, the objective was to develop a final chelating agent, with antiseptic power superior to that of EDTA. In stage 2, to be developed in England, the researcher will evaluate the interface between dentin/sealer and elucidate the better irrigation protocol, including the use of solution of stage one, to promote enhanced sealing Materials and Methods: Stage 1: the best chelating agent was selected, considering smear layer removal; antimicrobial capacity against in vitro Enterococcus faecalis biofilm and its adhesion to dentin, wettability, push-out test and cytotoxicity. Stage 2: Different Irrigation protocols: NaOCl/saline/sealer; NaOCl/17% EDTA/sealer; NaOCl/17%EDTA/Benzychonium chloride/sealer will be done before the obturation of root canal with the sealers: MTA Fillapex, BioRoot RCS, Totalfill. After we will proceed with the experiments. (n=3) For Confocal Laser Scanning, fluo3 dye powder will be mixed with the sealers and the interface micropermeability of specimens will be investigated by obtained images. For Scanning Electron Microscopy the microstructural characterization of dentin/sealer will be analyzed. For Atomic Force Microscopy a thin probe with atomic termination will sweeps dentin/sealer surface topography. For Micro Raman spectroscopy a laser source (25 mW line illumination) will be used into in-house curve-fitting software for mapping the dentin and cement (in groups that are present) characteristic peaks and calculate the diffusion lengths. (AU)