Monosodium urate and silibinin modulation of monocytes from preeclamptic women and THP-1 lineage of human monocytes

• Grant number: 17/26053-4
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): May 01, 2018
• Effective date (End): February 29, 2020
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Terezinha Serrão Peraçoli
• Grantee: Virgínia Juliani Gomes
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 19/10250-0 - Effects of supernatant from monocytes of pre-eclamptic women treated with sibilin on oxidative stress and nitric oxide availability in endothelial cells, BE.EP.MS

Abstract

Preeclampsia (PE) is a gestational specific syndrome characterized by a maladaptation state of the immunological tolerance, identified by oxidative stress and abnormal activation of the innate immune system. In plasma of pregnant women with PE there are elevated levels of molecular structures associated with stress and cell death, called damage-associated molecular patterns (DAMPs) such as uric acid, which appear to contribute directly to the pathogenesis of this disease. These DAMPs can activate monocytes switching them to the M1 profile. The imbalance between pro- and anti-inflammatory states in PE may be improved by administration of silibinin, a flavonoid with anti-inflammatory properties. This work aims to evaluate the immunomodulatory effect of silybinin on the expression of inflammatory markers and on the nuclear transcription factor B (NF-kB) signaling pathway in monocytes from pregnant women with PE and in THP-1 lineage of human monocytes activated with MSU.Monocytes from pregnant women with PE will be cultured with or without silibinin and THP-1 cells will be cultured with or without monosodium urate (MSU), and/or silibinin for different periods: a) 30 min for analysis of protein expression of IKB-± and NF-kBp65 by flow cytometry, b) 18 h for expression analysis of the surface markers TLR4, CD64, CD163 and CD14 and for determination of IL-1², IL-6, IL-8, IL-10, IL-12p70 and TNF-± by flow cytometry, and IL-23 and TGF-² concentrations by ELISA. The results will be analyzed using parametric or nonparametric tests with 5% of significance level. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, VIRGINIA JULIANI; NUNES, PRISCILA REZECK; MATIAS, MARIANA LETICIA; RIBEIRO, VANESSA ROCHA; DEVIDES, AMANDA CARREIRA; BANNWART-CASTRO, CAMILA FERREIRA; ROMAGNOLI, GRAZIELA GORETE; PERACOLI, JOSE CARLOS; SERRAO PERACOLI, MARIA TEREZINHA; ROMAO-VEIGA, MARIANA. Silibinin induces in vitro M2-like phenotype polarization in monocytes from preeclamptic women. International Immunopharmacology, v. 89, p. 9-pg., 2020-12-01. (17/26053-4, 16/18155-9)

GOMES, VIRGINIA JULIANI; NUNES, PRISCILA REZECK; MATIAS, MARIANA LETICIA; RIBEIRO, VANESSA ROCHA; DEVIDES, AMANDA CARREIRA; BANNWART-CASTRO, CAMILA FERREIRA; ROMAGNOLI, GRAZIELA GORETE; PERACOLI, JOSE CARLOS; SERRAO PERACOLI, MARIA TEREZINHA; ROMAO-VEIGA, MARIANA. Silibinin induces in vitro M2-like phenotype polarization in monocytes from preeclamptic women. International Immunopharmacology, v. 89, n. A, DEC 2020. (17/26053-4, 16/18155-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GOMES, Virgínia Juliani. Effects of monosodium urate and silibinin on monocytes of preeclamptic women in vitro polarization. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina. Botucatu Botucatu.