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Characterization of the microbiological and endotoxic profile of patients with chronic periodontitis and secondary endodontic involvement submitted to the endodontic therapy

Grant number: 17/18838-1
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2018
Effective date (End): February 28, 2019
Field of knowledge:Health Sciences - Dentistry - Endodontics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Lidiane Mendes Louzada
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated research grant:15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections, AP.TEM


Although there is an extensive literature on the microbiota associated with periodontal lesions and on the one associated with endodontic lesions, few studies have focused on the investigation of the microbiota of the combined endoperiodontal lesions (EPL), particularly of teeth with chronic periodontal disease and secondary endodontic involvement. The objectives of the present study are: 1) To detect the presence, levels and proportions of specific bacterial species in root canals (RC) and periodontal pockets (PP) of teeth with teeth with chronic periodontal disease and secondary endodontic involvement; 2) To investigate and quantify the presence of endotoxins (LPS) and lipoteicoic acid (LTA) in RC and PP; 3) To correlate specific microorganisms (MO) and LPS / LTA levels with the clinical and radiographic aspects of the involved teeth. Ten patients with vital pulp and associated PP, characterizing endoperiodontal involvement, will be selected. Samples will be collected with sterile / pyrogenic paper points from RC and PP and frozen for future analysis. The MO of both sites will be identified using molecular techniques such as Nested-PCR and checkerboard. The quantification of LPS and LTA in both sites will be performed using the LAL and ELISA methods, respectively. All the results obtained, as well as the clinical and radiographic aspects of the involved teeth, will be tabulated in Excel and later analyzed statistically by specific software. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LOUZADA, LIDIANE M.; ARRUDA-VASCONCELOS, RODRIGO; DUQUE, THAIS M.; CASARIN, V, RENATO C.; FERES, MAGDA; GOMES, BRENDA P. F. A. Clinical Investigation of Microbial Profile and Levels of Endotoxins and Lipoteichoic Acid at Different Phases of the Endodontic Treatment in Teeth with Vital Pulp and Associated Periodontal Disease. JOURNAL OF ENDODONTICS, v. 46, n. 6, p. 736-747, JUN 2020. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LOUZADA, Lidiane Mendes. Clinical study of the microbial profile, LPS and LTA in teeth with pulp vitality associated with periodontal disease. 2019. 207 f. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba.

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