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Evaluation of gene associated with muscular development by gene edition (CRISPR/Cas9)

Grant number: 17/25119-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2018
Status:Discontinued
Field of knowledge:Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
Principal Investigator:Luiz Lehmann Coutinho
Grantee:Bruna Petry
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:14/08704-0 - Identification of loci of interest for poultry production, AP.TEM
Associated scholarship(s):19/14876-1 - Functional analysis of the SAP30 gene and its relation to muscle development, BE.EP.DR

Abstract

The intense animal breeding in poultry production has brought advances in poultry production, improving commercial value and and reducing production costs. New methodologies have been used to identify genes and/or genetic variations that are candidates for the regulation of traits of economic interest in chickens. However, despite advances in the technologies employed, the accurately establishing of the effects that genetic variants have on the phenotype, still a challenge. In this context, the use of a genome-editing technique (CRISPR/Cas9), which, through the knockout or editing of genes and mutations validates the effect of these changes on the phenotype, will provide advances in the understanding of the regulation of phenotypic traits in broiler chickens. Moreover, the main goal of this project is to identify candidate genes and/or mutations for muscle deposition regulation in broiler chicken lines and to test the effect of this gene in muscle cell cultures through gene editing. (AU)