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Influence of bioregulators on oxidative stress caused by cadmium in Glycine max

Grant number: 17/12349-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2018
Effective date (End): December 31, 2018
Field of knowledge:Biological Sciences - Botany
Principal Investigator:Suzana Chiari Bertolli
Grantee:Guilherme Sanches Francischini
Home Institution: Faculdade de Artes, Ciências, Letras e Educação de Presidente Prudente (FACLEPP). Universidade do Oeste Paulista (UNOESTE). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil

Abstract

Soybean cultivation in contaminated soil by heavy metals can result in plants with altered growth, productivity and quality of beans. This may occur due to increased production of reactive oxygen species (ROS) caused by the high concentration of these metals, and can generate damage at different levels of plant organization and at different stages of development. On the other hand, ROS production is related to the action of plant bioregulators, since these may have a strong relation with the balance link of ROS levels, directing their role in generating oxidative damages or signaling in plants under stress. However, in general, there is no accurate information on how the bioregulators influence specific physiological processes, especially those related to oxidative stress caused by heavy metals. In this sense, the objective of this project is to evaluate the effects of cadmium (Cd) on the development and physiological responses of soybean seedlings and verify the potential attenuating effect of oxidative stress by the exogenous application of plant bioregulators, with the hypothesis that the seedlings growth and the antioxidant activity will be potentialized by the application of bioregulators in situations of different Cd. Glycine max (L.) seeds will be sowed on a Mangelsdorf chamber with different concentrations of Cd and application of Stimulate® (product composed by auxin, gibberellin and abscisic acid). After seven days in development, seedlings are collected and the biometric, biochemical parameters and Cd tolerance index of shoot and root will be analyzed. The results of each variable were analyzed by analysis of variance (ANOVA, p <0.05), and as means are compared by the Tukey test at 5% probability.