Effect of caloric restriction on isolated pancreatic islets on cell coupling, impact of electrical activity and intracellular Ca 2+ oscillation

• Grant number: 18/04536-6
• Support Opportunities: Scholarships abroad - Research
• Effective date (Start): July 30, 2018
• Effective date (End): July 29, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Maria Esméria Corezola do Amaral
• Grantee: Maria Esméria Corezola do Amaral
• Host Investigator: Richard Benninger
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Centro Universitário Hermínio Ometto (UNIARARAS). Fundação Hermínio Ometto (FHO). Araras , SP, Brazil
• Research place: University of Colorado, Denver (CU), United States

Abstract

Intercellular communication mediated by gap junctions contributes to appropriate insulin secretion. Data from the literature show that connexin (Cx) 36 is closely related to insulin secretion by glucose stimulation or other segretagogues. However, there are few studies in the literature that characterize the functionality of the Cx and the relationship of this coupling with the electrical pulsatility through the oscillations of [Ca2+] so that insulin secretion occurs adequately associated with the feeding intervention. The objective of the present study is to evaluate the effect of caloric restriction (CR) on intercellular coupling in isolated pancreatic islets and the impact of electrical activity and intracellular oscillations of [Ca2+] on the endocrine function of obese mice. This study will allow the understanding of the intercellular communication of animals submitted to CR characterized by low circulating insulin and increased peripheral sensitivity to insulin. It will also provide the possibility of understanding the pathologies, such as obesity and metabolic syndrome, related to the functioning of the communicating channels and insulin secretion, as well as to suggest ways of interventions to minimize or delay the onset of these pathologies.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMARAL, MARIA C.; KRAVETS, VIRA; DWULET, JAEANN M.; FARNSWORTH, NIKKI L.; PISCOPIO, ROBERT A.; SCHLEICHER, WOLFGANG; MIRANDA, JOSE; BENNINGER, RICHARD K.. Increased Gap Junction Coupling in the Islet of Langerhans following Caloric Restriction. Diabetes, v. 69, p. 2-pg., 2020-06-01. (18/04536-6)

COREZOLA DA AMARAL, MARIA ESMERIA; KRAVETS, VIRA; DWULET, JAEANN M.; FARNSWORTH, NIKKI L.; PISCOPIO, ROBERT; SCHLEICHER, WOLFGANG E.; MIRANDA, JOSE GUADALUPE; BENNINGER, RICHARD K. P.. Caloric restriction recovers impaired beta-cell-beta-cell gap junction coupling, calcium oscillation coordination, and insulin secretion in prediabetic mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, v. 319, n. 4, p. E709-E720, OCT 2020. (18/04536-6)