Toxicogenomic potential of environmental contaminant 2-phenylbenzotriazole 9 (PBTA-9) in vivo

• Grant number: 18/04105-5
• Support type: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2018
• Effective date (End): May 31, 2020
• Field of knowledge: Biological Sciences - Genetics
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Daisy Maria Favero Salvadori
• Grantee: Amanda Rodrigues Tanamachi
• Home Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The constantly expanding industrial sector has been generating daily residues with high toxic potential to the environment. The textile industry has a heavily impact polluting the hydrosphere due to the compounds and chemical processes used. Literature shows that even after effluent treatment toxic compounds are still present in the wastewater and in rivers. Moreover, the water chlorination for human consume can make some dyes compounds even more toxics. Currently, environmental pollution caused by azo dyes, their byproducts and intermediates, has been widely investigated. In this sense, the non-chlorinated PBTA-9 (2-fenilbenzotriazol 9) has received attention because it is derived from the dye Disperse Violet 93, which is detected in high quantity in surface waters under influence of textile activities. Therefore, the aim of this study is to investigate the toxicogenomic potential of acute exposure to the non-chlorinated PBTA-9 in mice. One of the three doses (5, 50 and 500 ¼g/kg body weight) of the compound will be orally (gavage) administered to the animals. Micronucleus frequency in bone marrow cells, primary DNA damage in liver and colon cells, and gene (TP53, GSTP1 and CDKN1A) expression of in liver cells will be analyzed. We hope the results can be used for risk assessment concerning human exposure to PBTA-9. This study is part of the Research Project "National Institute of Alternative Technologies for Detection, Toxicological Assessment and Removal of Micropollutants and Radioactive" (INCT-DATREM). (AU)