The insulin-like growth factor (IGF), together with its binding protein (IGFBP), participate in lipid metabolism and adiposity regulation. A good regulator for this set is Pregnancy-Associated Plasma Protein A (PAPP-A) capable of breaking the bonds between IGF and its binding proteins increasing the bioavailability of free IGF, which leads to a reduction in the amount of lipids due to the increase in the rate of ² oxidation. Many studies have already investigated the action of exogenous IGF-I on in vitro maturation (IVM), but little was known about the effects of PAPP-A addition during this step; and its impact on quality and future embryonic metabolism. Recent data obtained by our group report that the addition of 100 ng/mL of PAPP-A during IVM has positive effects to embryo quality. Thus, evaluate the addition or modulation of the IGF composition and its availability are important researches. In this context, the objective of this research project is to investigate the effects of adding 100 ng/mL PAPP-A during IVM of cumulus-oocyte complexes (COCs) from slaughterhouse ovaries about: 1) the lipid content of blastocysts, by means of the Sudan-Black B coloration; 2) the phospholipid profile of blastocysts, through mass spectrometry analysis (MALDI-MS); and 3) the pattern of genes expression related to the lipid profile in embryos.
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