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Impact of pregnancy-associated plasma protein- a (PAPP-A) addition during in vitro oocyte maturation on the profile and lipid metabolism of bovine embryos

Grant number: 18/06454-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2018
Effective date (End): November 30, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Anthony César de Souza Castilho
Grantee:Victor Augusto Vieira de Lima
Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
Associated research grant:13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression, AP.JP


The insulin-like growth factor (IGF), together with its binding protein (IGFBP), participate in lipid metabolism and adiposity regulation. A good regulator for this set is Pregnancy-Associated Plasma Protein A (PAPP-A) capable of breaking the bonds between IGF and its binding proteins increasing the bioavailability of free IGF, which leads to a reduction in the amount of lipids due to the increase in the rate of ² oxidation. Many studies have already investigated the action of exogenous IGF-I on in vitro maturation (IVM), but little was known about the effects of PAPP-A addition during this step; and its impact on quality and future embryonic metabolism. Recent data obtained by our group report that the addition of 100 ng/mL of PAPP-A during IVM has positive effects to embryo quality. Thus, evaluate the addition or modulation of the IGF composition and its availability are important researches. In this context, the objective of this research project is to investigate the effects of adding 100 ng/mL PAPP-A during IVM of cumulus-oocyte complexes (COCs) from slaughterhouse ovaries about: 1) the lipid content of blastocysts, by means of the Sudan-Black B coloration; 2) the phospholipid profile of blastocysts, through mass spectrometry analysis (MALDI-MS); and 3) the pattern of genes expression related to the lipid profile in embryos.

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