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Histopatologycal changes of the hamsters intestine experimentally infected by Leishmania (Leishmania) chagasi

Grant number: 18/04869-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2018
Effective date (End): May 31, 2019
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal Investigator:Renata de Britto Mari
Grantee:Sarah Kymberly Santos de Lima
Home Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

Visceral leishmaniasis (LV) is an infectious disease caused by the protozoan parasite belonging to the species Leishmania (L.) chagasi. This disease is of great importance for public health, because it affects wild animals, domestic animals and humans, mainly children whose immune system is not fully developed or individuals with acquired immunodeficiency virus (HIV), leading to death 90% of untreated cases. As the disease progresses, several organs are affected, including the spleen, liver and the gastrointestinal tract, causing a series of morph physiological and histopathological alterations, compromising the physiology of the infected organs, and, therefore, the breakdown of homeostasis. Thus, this work intends to investigate evolutionarily the histological changes that occur in the small and large intestine of golden hamsters (Mesocricetus auratus) infected by L. chagasi and to correlate them with the tissue parasitism. In this way, the animals will be infected experimentally by the intraperitoneal route with promastigotes forms of L. chagasi. After 30, 60 and 90 days of infection (PI), the animals will be euthanized, and the parasitism of the spleen, liver and intestine will be quantified through Leishman-Donovan units and immunohistochemistry. The intestinal histopathological changes will be evaluated using histological sections stained with hematoxylin-eosin (HE), periodic acid from Schiff (PAS) and alcian blue pH 2.5.