Advanced search
Start date
Betweenand

Detection of Chelonid Herpesvirus(ChHV-5) in blood and organs of Chelonia mydas (Testudines, Cheloniidae) and correlation with morphometrics in animals with and without fibropapillomatosis in the coast of Ubatuba/São Paulo

Grant number: 17/23309-8
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2018
Effective date (End): August 31, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Eliana Reiko Matushima
Grantee:Isabela Santos Silva
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Fibropapillomatosis (FP) is a benign neoplastic disease with epithelial origin characterized by simple or multiple skin tumors, verrucous or smooth texture, sessile or pedunculated. The size and number of lesions may compromise feeding, swimming, vision and physiological conditions, leading to chronic cases of stress and immunosupression. Chelonid herpesvírus-5 (ChHV-5) is considered the primary agent of this disease and has been detected in tumor, skin and organ samples. Several mechanisms on the etiology of ChHV-5 infection and clinical development of FP are poorly understood, particularly aspects related to infection and dissemination of the agent in the host organism. In order to detect the ChHV-5 and analyze its molecular characterization, samples of tumor, organs and blood of green sea turtles (Chelonia mydas) with and without FP captured in Ubatuba-SP region will be collected. The FP score system (FPS), morphometry (CCL - curved carapace length and BM-body mass) and body condition (good, regular, poor) will be determined, as well as the quality of the sea water of Ubatuba region. The polymerase chain reaction (PCR) technique will be applied and carried out using DNA polymerase genes, capsid proteins and membrane glycoproteins (region, UL18, UL22, UL27, GTHV, F-US3B). The data will be analyzed statistically in order to determine if there is a correlation between the presence of the virus and the described parameters. (AU)