Arterial rigidity, miRNA expression and eNOS polymorphism in patients with ischemic vascular brain accident by hypertensive emergency

• Grant number: 18/00119-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date until: May 01, 2018
• End date until: April 30, 2019
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: José Fernando Vilela Martin
• Grantee: Ana Luiza Possebon Mansor
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

Hypertensive crisis (CH) can cause lesions in target organs, characterizing hypertensive emergency. Highlighting the cerebrovascular accident (AVEi), among the possible factors related to CH, include arterial, polymorphisms of endothelial nitric oxide synthase (eNOS) and expression of micro-RNAs (miRNAs). Objective-To evaluate the association of arterial stiffness, polymorphismeNOS-G894T and miRNAs (221 and 222), with stroke resulting from hypertensive emergency. Casuistry and Methods - We will study 90 patients distributed in G1 - 30 with Hypertensive emergency; G2 - 30 individuals with non-emergency stroke hypertensive; G3 - 30 with controlled hypertension. The diagnosis of stroke will be performed by computed tomography of the skull. Will be collected blood sample analysis of genetic polymorphism and expression of micro-RNAs by RT-qPCR (real-time quantitative polymerase chain reaction). The clinical-demographic data will be obtained by application of a questionnaire. The arterial stiffness will be evaluated by brachial oscillometric monitor with evaluation of central hemodynamics, considering pulse wave velocity, central pressure and augmentation index. For Statistical analysis will be used the Kolmogorov-Smirnov tests, t-test or Mann Whitney, multiple linear regression and Pearson or Spearman. Will be admitted level of significance for p value <0.05.