Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance

• Grant number: 18/03924-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: November 01, 2018
• End date: August 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: André Furugen Cesar de Andrade
• Grantee: Mariana Andrade Torres
• Supervisor: Zoltan Machaty
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Purdue University, United States
• Associated to the scholarship: 16/09441-8 - Metabolomics sgnatures on the influence of holding time on increasing boar spermatozoa cryotolerance, BP.DR

Abstract

Despite great scientific investments, boar semen cryopreservation still presents an important challenge for science, regarding post-thawed boar semen quality, independently of pre-cryopreservation quality or cryopreservation protocol used. Some proteins have been described as freezability biological marks of boar semen. However, seminal plasma has a wide range of components, so it lead us to believe that not only proteins but also lipids, ions, and others components could be beneficial or detrimental to boar semen freezability. Jointly, holding time (incubation time in own seminal plasma before cryopreservation) has been used to improve post-thawed boar semen quality. Holding time allows greater contact of spermatozoa with seminal plasma components, and it is reported to improve cryotolerance at low temperatures. It led us to postulate whether cryopreservation with or without holding time changes the interaction between seminal plasma molecules and spermatozoa, and then freezability. Thus, three experiments will be conducted in order to understand the influence of the holding time on increasing boar spermatozoa cryotolerance. First experiment aims to determine theoptimal holding time to boar semen cryopreservation. Second experiment aims to study the relation between boar spermatozoa freezability and holding time. Finally, the third experiment aims to elucidate, through metabolomics which seminal molecules contributefor the increasing in boar spermatozoa freezability.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TORRES, MARIANA A.; PEDROSA, ANA CAROLINA; NOVAIS, FRANCISCO JOSE; ALKMIN, V, DIEGO; COOPER, BRUCE R.; YASUI, GEORGE S.; FUKUMASU, HEIDGE; MACHATY, ZOLTAN; DE ANDRADE, ANDRE F. C.. etabolomic signature of spermatozoa established during holding time is responsible for differences in boar sperm freezabilit. BIOLOGY OF REPRODUCTION, v. 106, n. 1, p. 213-226, JAN 2022. (16/09441-8, 18/03924-2, 16/24690-4)