The aim of this study is incorporate fluoride, or the combination of fluoride and tin chloride, encapsulated in nano tubes of halloysite and bentonite, into a hybrid coating and later test the anti-caries preventive potential of these films on enamel. Enamel specimens (5 mm x 5 mm), obtained from bovine teeth, will be prepared. The enamel specimens will be treated with the polymeric coatings (n=10), then they will have their surface divided into two parts (5 mm x 2,5 mm each), one where the treatments will be applied and then submitted to the cariogenic challenge (under treatment), and the other that will be submitted only to the cariogenic challenge (adjacent to treatment). After, all the treated specimens will be fixed vertically in metal devices, and immersed in 24-well culture plates to be submitted to a cariogenic challenge, using a biofilm model for 5 days. The response variable will be the mineral content change, measured by transverse microradiography, and expressed by the total mineral loss and average lesion depth. Data will be statistically analyzed using appropriate methods (± = 0.05).
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