Metabolomics characterization of sheep and cattle meat

• Grant number: 18/09800-3
• Support type: Scholarships abroad - Research Internship - Post-doctor
• Effective date (Start): October 31, 2018
• Effective date (End): October 30, 2019
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Saulo da Luz e Silva
• Grantee: Nara Regina Brandão Cônsolo
• Supervisor abroad: Linda Margareta Samuelsson
• Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Local de pesquisa : AgResearch, Christchurch, New Zealand
• Associated to the scholarship: 16/01320-7 - Metabolomic characterization of blood and meat from young Nellore bulls, progeny of bulls with different EPDs for growth, precocity and muscularity, BP.PD

Abstract

The aim of the project propose for the scholarship program is to improve the knowledge about nuclear magnetic resonance (NMR) spectral processing (including binning), metabolite identification and statistical analyses of semi-quantitative and quantitative NMR metabolomics data. The main activities that will be undertaken at AgResearch are dived in three phases: 1) Spectral processing and data analysis of data set from research project in Brazil. Around three hundred raw spectra of plasma and meat from Nellore bulls with divergent EPD for growth will be processed and statistically analyzed. 2) Confinement odour in New Zealand lamb meat. This study was conducted at AgResearch in 2016, and the meat from those animals will be analyze by NMR and statistical analyses of semi-quantitative and quantitative NMR metabolomics data will be performed. 3) Unlocking value of the whole carcass. This is other project that will be performed in the AgResearch and explore the investigating opportunities for underutilised parts of the carcass. The work includes metabolite profiling of parts of the carcass from several livestock classes, e.g. bobby calves, bull beef, sheep and culled dairy cows. The blood and meat metabolite analyses will use the high field nuclear magnetic resonance (NMR; Bruker Avance 600 NMR spectrometer; Bruker Corporation, Karlsruhe, Baden-Württemberg, Germany). The plasma and meat samples for the proton NMR analysis were prepared as specific methodologies, and data will be analyze mainly by Chenomix software. The results of three steps of this project is highly likely to be publishable in a journal like Meat Science. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONSOLO, NARA R. B.; OLIVECRONA, NATALIA; SAMUELSSON, LINDA M.; REIS, MARIZA G.; EDWARDS, PATRICK J. B.; REIS, MARLON M. Investigation of metabolites associated with confinement odour in chilled vacuum-packed lamb by proton nuclear magnetic resonance (H-1 NMR) spectroscopy. MEAT SCIENCE, v. 169, NOV 2020. Web of Science Citations: 0.

BRANDAO CONSOLO, NARA REGINA; DA SILVA, JULIANA; MACEDO BUARQUE, VICENTE LUIZ; HIGUERA-PADILLA, ANGEL; GARIBALDI SIMON BARBOSA, LUIS CARLOS; ZAWADZKI, ANDRESSA; COLNAGO, LUIS ALBERTO; NETTO, ARLINDO SARAN; GERRARD, DAVID EDWIN; SILVA, SAULO LUZ. Selection for Growth and Precocity Alters Muscle Metabolism in Nellore Cattle. METABOLITES, v. 10, n. 2 FEB 2020. Web of Science Citations: 0.