Effects of selenium supplementation in mice with hypercholesterolemia using statin

• Grant number: 18/09478-4
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2018
• End date: September 30, 2019
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Anderson Marliere Navarro
• Grantee: Lígia Moriguchi Watanabe
• Supervisor: Lucia Andreia Seale
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Hawaii at Manoa (UH), United States
• Associated to the scholarship: 16/05677-7 - Effect of brazil nut (Bertholetia excelsa H.B.K.) consumption on selenocompouds in chronic use of statins, BP.DR

Abstract

Statins inhibit the first steps of cholesterol biosynthesis in the mevalonate pathway. This pathway is also important for the maturation of selenocysteine tRNA (Sec tRNA), responsible for the expression of all selenoproteins, and, when inhibited, results in decreased availability of selenoproteins. The decrease in selenoproteins has been associated with side effects related to the use of statins such as myopathies and increased oxidative stress. Aim: The studies proposed herein will improve our understanding and insights regarding the effects of selenium supplementation combined with the use of statins in animal models. Methodology: Age-matched homozygous KO mice and wild-type (WT) homozygous littermates and/or C57BL/6J animals will be used in experiments. Blood samples will be collected for analysis of cholesterol levels, oxidative stress and creatine kinase activity. Total selenium content in the plasma, muscle and liver will be assessed by inductively-coupled plasma mass spectrometry (ICP-MS). Western Blot will be performed to quantify the expression of selenoproteins. Statistical tests will be used to assess differences between groups and the correlations between the variables of interest. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WATANABE, LIGIA M.; HASHIMOTO, ANN C.; TORRES, DANIEL J.; ALFULAIJ, NAGHUM; PERES, RAFAEL; SULTANA, RAZVAN; MAUNAKEA, ALIKA K.; BERRY, MARLA J.; SEALE, LUCIA A.. Effect of statin treatment in obese selenium-supplemented mice lacking selenocysteine lyase. Molecular and Cellular Endocrinology, v. 533, AUG 1 2021. (18/09478-4)

SEALE, LUCIA A.; OGAWA-WONG, ASHLEY N.; WATANABE, LIGIA M.; KHADKA, VEDBAR S.; MENOR, MARK; TORRES, DANIEL J.; CARLSON, BRADLEY A.; HATFIELD, DOLPH L.; BERRY, MARLA J.. Adaptive Thermogenesis in a Mouse Model Lacking Selenoprotein Biosynthesis in Brown Adipocytes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 2, JAN 2021. (18/09478-4)

SEALE, LUCIA A.; KHADKA, VEDBAR S.; MENOR, MARK; XIE, GUOXIANG; WATANABE, LIGIA M.; SASUCLARK, ALEXANDRU; GUIRGUIS, KYRILLOS; HA, HERENA Y.; HASHIMOTO, ANN C.; PEPLOWSKA, KAROLINA; et al. Combined Omics Reveals That Disruption of the Selenocysteine Lyase Gene Affects Amino Acid Pathways in Mice. NUTRIENTS, v. 11, n. 11, NOV 2019. (18/09478-4)