Matabolic engineering of Saccharomyces cerevisiae wild strains using the CRISPR/Cas9 system

• Grant number: 18/03403-2
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): June 01, 2018
• Effective date (End): November 30, 2020
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Gonçalo Amarante Guimarães Pereira
• Grantee: Carla Maneira da Silva
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 19/05339-2 - Use of CRISPR / dCas9 and microfluidics to uncover gene interactions related to the conversion of xylose into ethanol by S. cerevisiae industrial strains, BE.EP.MS

Abstract

Over the last few decades, the demand for renewable energy has fueled efforts by research institutions and industries to produce hemicellulosic (or second generation) (E2G) ethanol. However, the economic viability of this process depends on the development of commercial strains of Saccharomyces cerevisiae capable of converting five carbons sugars into ethanol, besides tolerating stresses common to the industrial condition. To achieve these goals, metabolic engineering is necessary and, in this context, yeasts adapted to the industrial conditions - industrial yeasts - are considered appropriate backgrounds because they tolerate different stresses inherent to different processes. Although S. cerevisiae is known as an easy subject for genetic engineering, wild strains are usually diploid or polyploid, and therefore require the development or adaptation of efficient genetic manipulation tools, such as the CRISPR / Cas9 genomic editing system. In this project, the main objective is to evaluate and establish a minimum set of genetic modifications necessary to obtain S. cerevisiae diploid lines capable of satisfactorily converting xylose into ethanol. Thus, the metabolic engineering approach based on the CRISPR / Cas9 system intends to enable the development of lineages for testing and application in the E2G process requiring significantly less time compared to traditional techniques. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORADINI, V, ALESSANDRO L.; BEZERRA DE MELLO, FELLIPE DA SILVEIRA; FURLAN, MONIQUE; MANEIRA, CARLA; CARAZZOLLE, MARCELO F.; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae. BIOTECHNOLOGY FOR BIOFUELS, v. 14, n. 1, DEC 16 2021. (16/12852-0, 16/02506-7, 15/06677-8, 14/26719-4, 18/03403-2, 13/08293-7)

MANEIRA, CARLA; BERMEJO, PAMELA MAGALI; GUIMARAES PEREIRA, GONCALO AMARANTE; BEZERRA DE MELLO, FELLIPE DA SILVEIRA. Exploring G protein-coupled receptors and yeast surface display strategies for viral detection in baker's yeast: SARS-CoV-2 as a case study. FEMS Yeast Research, v. 21, n. 1, FEB 2021. (18/03403-2)