Erythromycin in aquaculture: studies for drug incorporation in feed and for residual depletion in fish

• Grant number: 18/05536-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date until: August 01, 2018
• End date until: February 29, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Jonas Augusto Rizzato Paschoal
• Grantee: Sarah Chagas Campanharo
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 12/18334-0 - Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study, AP.JP

Abstract

Aquaculture is an intensive system dedicated to production of aquatic animals such as fish and crustaceans, this practice generates more homogenous and traceable products, but represents an environment favorable to spread diseases. In Brazil, aquaculture is growing rapidly. Although there is a growing market for animal health products and veterinary drugs, there are few options for regulated medicines for use in aquaculture in Brazil, which may lead to inappropriate use of products and substances. In this view, there is a scenario of great demand for new therapeutic alternatives that are effective against the major diseases that affect aquaculture species of commercial interest for Brazil and that may be regulated for veterinary use in this sector. Erythromycin is a macrolide antimicrobial, widely known and used in human and veterinary medicine, which acts by interfering with bacterial protein synthesis. This drug has a wide activity against gram-positive bacteria, such as streptococci, staphylococci and gram-positive diplococci, with at least three Streptococcus species causing outbreaks with great losses and economic impact in Brazil. Although erythromycin is a high potency antibiotic, its use in fish farming is not regulated in Brazil yet, although it is already approved for use in other countries. In this project, it is proposed to carry out a residual depletion study of erythromycin in pacu after oral administration aiming at estimating a minimum withdrawal time to be recommended, considering food safety issues. To do so, a study of drug incorporation in the feed will also be carried out aiming at obtaining a homogenous process and capable of avoiding or mitigating the risk of leaching of the drug from the feed to the aquatic environment. Furthermore, an analytical method will be developed employing the promising dispersive liquid-liquid microextraction technique in the sample preparation stage and the high efficiency liquid chromatography coupled to mass spectrometry. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, JONAS JOAQUIM MANGABEIRA; CAMPANHARO, SARAH CHAGAS; PASCHOAL, JONAS AUGUSTO RIZZATO. Ethnoveterinary for food-producing animals and related food safety issues: A comprehensive overview about terpenes. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, v. 20, n. 1, p. 48-90, JAN 2021. (19/06159-8, 17/15052-7, 18/05536-0, 12/18334-0)

DE FREITAS, LUCAS VICTOR PEREIRA; DA MOTA, CARLOS AUGUSTO ALVARENGA; DAMACENO, MARINA ALVES; SILVEIRA, JULIANA GRELL FERNANDES; PORTELA, ANA CAROLINA VELLOSA; CAMPANHARO, SARAH CHAGAS; DA SILVA, AGNALDO FERNANDO BALDO; ASSANE, INACIO MATEUS; PILARSKI, FABIANA; SASANYA, JAMES JACOB; et al. Residue Depletion Profile and Estimation of Withdrawal Period for Sulfadimethoxine and Ormetoprim in Edible Tissues of Nile Tilapia (Oreochromis sp.) on Medicated Feed. ANIMALS, v. 13, n. 15, p. 13-pg., 2023-08-01. (21/03294-1, 19/06159-8, 16/16593-9, 18/05536-0, 21/08152-0, 19/22775-0, 12/18334-0)

ASSANE, INACIO MATEUS; DAMACENO, MARINA ALVES; CAMPANHARO, SARAH CHAGAS; DA SILVA, AGNALDO FERNANDO BALDO; DE SOUSA, ELIELMA LIMA; OLIVEIRA, ANDRE DO VALE; FERREIRA, DANIEL DE ABREU REIS; KOTZENT, SUZANA; DE JESUS, RAPHAEL BARBETTA; DA PAZ, DEBORAH JACOB FREIRE; et al. Thiamphenicol and florfenicol combination in Nile tilapia: Simultaneous detection and quantification in plasma and muscle plus skin samples, and pharmacokinetics following single oral administration. Aquaculture, v. 577, p. 11-pg., 2023-12-15. (18/05536-0, 19/22775-0, 19/06159-8)

CAMPANHARO, SARAH CHAGAS; DA SILVA, AGNALDO FERNANDO BALDO; BLEUZEN, ANAIS; DA SILVA, JONAS JOAQUIM MANGABEIRA; DE FREITAS, LUCAS VICTOR PEREIRA; ASSANE, INACIO MATEUS; PILARSKI, FABIANA; PASCHOAL, JONAS AUGUSTO RIZZATO. The association of modified QuEChERS and DLLME to offer high analytical detectability to assess residual depletion profile of erythromycin in fish. Food Chemistry, v. 405, p. 8-pg., 2023-03-30. (12/18334-0, 19/06159-8, 17/15052-7, 18/05536-0, 19/22775-0, 21/03294-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAMPANHARO, Sarah Chagas. Development of an analytical method using modified QuEChERS and DLLME for the determination of erythromycin residues in fish by LC-MS/MS. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.