Study about Flavivirus epitopes with medical importance in Brazil and its antibodies

• Grant number: 18/09383-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2018
• End date: January 31, 2022
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Luiz Tadeu Moraes Figueiredo
• Grantee: Marcilio Jorge Fumagalli
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/02438-6 - Studies with Bunyaviridae that produce human disease, AP.TEM

Abstract

In Brazil, there are important arboviruses capable of producing human disease, such as Dengue virus (DENV), Yellow Fever virus (YFV) and Zika Virus (ZIKV). They are transmitted by the bite of hematophagous mosquitoes, mainly of the genus Aedes, present in practically the whole Brazilian territory. The symptoms developed by DENV or ZIKV infection include abrupt fever, joint and muscle pain. In yellow fever, the symptoms may be more severe, including fulminant hepatitis, renal failure, hemorrhage and shock. Dengue fever and yellow fever may develop as high lethality haemorrhagic fevers and the Zika virus can cause severe congenital disease. Therefore, these viruses that are causing epidemics in Brazil, are of great importance for public health, requiring vigorous research and action for control. Considering the relevance of such arboviruses, we propose to study epitopes in Flavivirus proteins, mapping them with a microarray chip, a new technology. After recognition, these epitopes will be targets of studies that will include its usage in diagnosis methods of diagnosis and production of monoclonal antibodies for acute phase diagnosis, immunopathology studies, analysis of antibodies directed against the epitopes studied for viral neutralizing capacity or ADE (Antibody Dependent Enhancement). Also, epitopes will be tested as vaccine candidates and antibodies as therapeutic candidates. Eventually, the antigenic epitopes of Flaviviruses discovered or the antibodies raised against them, may become commercial products and be patented. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; CARRERA, JEAN PAUL; DE ARAUJO, JANSEN; CARDOSO, JEDSON FERREIRA; DE CARVALHO, CRISTIANO; DURIGON, EDISON LUIZ; QUEIROZ, LUZIA HELENA; FARIA, NUNO RODRIGUES; MURCIA, PABLO R.; et al. Paramyxoviruses from neotropical bats suggest a novel genus and nephrotropism. INFECTION GENETICS AND EVOLUTION, v. 95, NOV 2021. (15/05778-5, 19/24251-9, 13/14929-1, 18/09383-3, 17/13981-0, 18/14389-0)

NASTRI, ANA CATHARINA; DUARTE-NETO, AMARO NUNES; CASADIO, LUCIANA VILAS BOAS; SOUZA, WILLIAM MARCIEL DE; CLARO, INGRA M.; MANULI, ERIKA R.; SELEGATTO, GLORIA; SALOMA, MATIAS C.; FIALKOVITZ, GABRIEL; TABORDA, MARIANE; et al. Understanding Sabia virus infections (Brazilian mammarenavirus). TRAVEL MEDICINE AND INFECTIOUS DISEASE, v. 48, p. 9-pg., 2022-07-01. (18/14389-0, 18/09383-3)

FUMAGALLI, MARCILIO JORGE; MORAES FIGUEIREDO, LUIZ TADEU; AQUINO, VICTOR HUGO. Linear and Continuous Flavivirus Epitopes From Naturally Infected Humans. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, AUG 12 2021. (19/26119-0, 18/09383-3, 19/27333-6)

FUMAGALLI, MARCILIO JORGE; CASTRO-JORGE, LUIZA ANTUNES; DE SOUZA, WILLIAM MARCIEL; DE AZEVEDO, PATRICK ORESTES; HANSEN, ALANA WITT; GAZZINELLI, RICARDO TOSTES; DA FONSECA, BENEDITO ANTONIO LOPES; SPILKI, FERNANDO ROSADO; FIGUEIREDO, LUIZ TADEU MORAES. oronaVac and ChAdOx1 Vaccination and Gamma Infection Elicited Neutralizing Antibodies against the SARS-CoV-2 Delta Varian. IRUSES-BASE, v. 14, n. 2, FEB 2022. (18/09383-3, 20/05527-0, 19/26119-0)

CANDIDO, DARLAN S.; CLARO, INGRA M.; DE JESUS, JAQUELINE G.; SOUZA, WILLIAM M.; MOREIRA, FILIPE R. R.; DELLICOUR, SIMON; MELLAN, THOMAS A.; DU PLESSIS, LOUIS; PEREIRA, RAFAEL H. M.; SALES, FLAVIA C. S.; et al. Evolution and epidemic spread of SARS-CoV-2 in Brazil. Science, v. 369, n. 6508, SI, p. 1255+, SEP 4 2020. (20/04558-0, 16/00194-8, 19/21301-5, 18/14389-0, 18/14933-2, 17/13981-0, 20/04836-0, 18/25468-9, 16/18445-7, 18/09383-3, 18/17176-8, 19/12000-1, 19/07544-2, 19/24251-9)

FUMAGALLI, MARCILIO JORGE; DE SOUZA, WILLIAM MARCIEL; DE CASTRO-JORGE, LUIZA ANTUNES; HOMEM DE CARVALHO, RENAN VILLANOVA; CASTRO, ITALO DE ARAUJO; NOGUEIRA DE ALMEIDA, LUIZ GUSTAVO; CONSONNI, SILVIO ROBERTO; ZAMBONI, DARIO SIMOES; MORAES FIGUEIREDO, LUIZ TADEU. Chikungunya Virus Exposure Partially Cross-Protects against Mayaro Virus Infection in Mice. Journal of Virology, v. 95, n. 23, DEC 2021. (19/27333-6, 17/13981-0, 18/09383-3)

FUMAGALLI, MARCILIO JORGE; CAPATO, CARLOS FABIANO; DE CASTRO-JORGE, LUIZA ANTUNES; DE SOUZA, WILLIAM MARCIEL; ARRUDA, EURICO; FIGUEIREDO, LUIZ TADEU MORAES. Stability of SARS-CoV-2 and other airborne viruses under different stress conditions. ARCHIVES OF VIROLOGY, v. 167, n. 1, NOV 2021. (18/09383-3, 19/26119-0, 19/27333-6)

SILVA DE LIMA, SHIRLENE TELMOS; DE SOUZA, WILLIAM MARCIEL; CAVALCANTE, JOHN WASHINGTON; CANDIDO, DARLAN DA SILVA; FUMAGALLI, MARCILIO JORGE; CARRERA, JEAN-PAUL; SIMOES MELLO, LEDA MARIA; DE CARVALHO ARAUJO, FERNANDA MONTENEGRO; CAVALCANTE RAMALHO, IZABEL LETICIA; DE ALMEIDA BARRETO, FRANCISCA KALLINE; et al. Fatal Outcome of Chikungunya Virus Infection in Brazil. Clinical Infectious Diseases, v. 73, n. 7, p. E2436-E2443, OCT 1 2021. (18/00837-1, 17/13981-0, 18/09383-3, 19/24251-9)

FUMAGALLI, MARCILIO JORGE; CASTRO-JORGE, LUIZA ANTUNES; FRAGA-SILVA, THAIS FERNANDA DE CAMPOS; DE AZEVEDO, PATRICK ORESTES; CAPATO, CARLOS FABIANO; RATTIS, BRUNA AMANDA CRUZ; HOJO-SOUZA, NATALIA SATCHIKO; FLORIANO, VITOR GONCALVES; DE CASTRO, JULIA TEIXEIRA; RAMOS, SIMONE GUSMAO; et al. Protective Immunity against Gamma and Zeta Variants after Inactivated SARS-CoV-2 Virus Immunization. Viruses-Basel, v. 13, n. 12, DEC 2021. (19/26119-0, 20/05527-0, 18/09383-3, 19/09881-6, 19/11213-1)

LEE, QUINTIN; PADULA, MATTHEW P.; PINELLO, NATALIA; WILLIAMS, SIMON H.; O'ROURKE, MATTHEW B.; FUMAGALLI, MARCILIO JORGE; ORKIN, JOSEPH D.; SONG, RENHUA; SHABAN, BABAK; BRENNER, ORI; et al. Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys. PLOS PATHOGENS, v. 16, n. 1, JAN 2020. (17/13981-0, 18/09383-3)

DE SOUZA, WILLIAM MARCIEL; FUMAGALLI, MARCILIO JORGE; DE ARAUJO, JANSEN; OMETTO, TATIANA; MODHA, SEJAL; THOMAZELLI, LUCIANO MATSUMIYA; DURIGON, EDISON LUIS; MURCIA, PABLO RAMIRO; MORAES FIGUEIREDO, LUIZ TADEU. Discovery of novel astrovirus and calicivirus identified in ruddy turnstones in Brazil. SCIENTIFIC REPORTS, v. 9, APR 3 2019. (11/13821-7, 12/24150-9, 17/13981-0, 16/01414-1, 15/05778-5, 18/09383-3, 13/05485-2, 13/14929-1, 09/05994-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FUMAGALLI, Marcilio Jorge. Studies on aspects of the immune response in viral infections including: cross-protection between Chikungunya and Mayaro viruses; analysis of Zika virus E protein antigenic epitopes; and evaluation of response to inactivated SARS-CoV-2 vaccine. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.