Development of bioreactors for online studies of drug metabolism

• Grant number: 18/03035-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: July 01, 2018
• End date: February 28, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Quezia Bezerra Cass
• Grantee: Izadora Liranço Furlani
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock, AP.PCPE
• Associated scholarship(s): 21/10410-8 - Metabolomic and lipidomic approaches in neurotoxicity studies using cell culture models, BE.EP.DD

Abstract

In the early stages of the development of new drugs the study of in vitro metabolism is essential to understand the metabolic profile of the new chemical entity. The studies are traditionally carried out in solution, requiring a large number of tests to trace this profile, which results in the use of large volumes of microsomal fraction, cofactors and sample preparation time for further analysis. Thus, novel analytical strategies that minimize costs and increase test efficiency are of paramount importance for in vitro drug metabolism studies.In this scenario, the present project aims to develop a model of bioanalytical assays through the immobilization of microsomal fractions in capillary supports of fused silica and magnetic particles. Through the developed prototype the in vitro metabolism assays can be automated, thus simplifying the characterization of the metabolites produced. In addition, the test model meets the demands of green chemistry regarding the reduction of waste generated, low consumption of biological material, and reuse of the enzymatic system in various analyzes. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LONG, SOLIDA; FURLANI, IZADORA L.; DE OLIVEIRA, JULIANA M.; RESENDE, DIANA I. S. P.; SILVA, ARTUR M. S.; GALES, LUIS; PEREIRA, JOSE A.; KIJJOA, ANAKE; CASS, QUEZIA B.; OLIVEIRA, REGINA V.; et al. Determination of the Absolute Configuration of Bioactive Indole-Containing Pyrazino[2,1-b]quinazoline-3,6-diones and Study of Their In Vitro Metabolic Profile. Molecules, v. 26, n. 16, AUG 2021. (18/03035-3, 20/05965-8, 19/15040-4)

DE LIMA, JULIANA MARIA; FURLANI, IZADORA LIRANCO; GUIMARAES SILVA, LARISSA RAMOS; VALVERDE, ALESSANDRA LEDA; CASS, QUEZIA BEZERRA. Micro- and nano-sized amine-terminated magnetic beads in a ligand fishing assay. ANALYTICAL METHODS, v. 12, n. 33, p. 4116-4122, SEP 7 2020. (14/50249-8, 18/03035-3)

IZADORA L. FURLANI; BRUNO S. AMARAL; REGINA V. OLIVEIRA; QUEZIA B. CASS. Imobilização enzimática: conceito e efeitos na proteólise. Química Nova, v. 43, n. 4, p. 463-473, 2020-06-10. (18/03035-3, 13/01710-1, 14/50249-8, 15/25755-0)

FURLANI, IZADORA LIRANCO; OLIVEIRA, REGINA VINCENZI; CASS, QUEZIA BEZERRA. Glucose-6-phosphate dehydrogenase immobilized onto magnetic beads (G6PDH-Mb) as a generator system for production of NADPH: Development and application in metabolism studies. Journal of Pharmaceutical and Biomedical Analysis, v. 219, p. 9-pg., 2022-06-30. (20/05965-8, 14/50249-8, 18/03035-3)