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Stress and periodontitis: evaluation of sympathetic innervation in the periodontium and the influence of noradrenaline on Porphyromonas gingivalis in Galleria mellonella

Grant number: 17/26461-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2018
Effective date (End): January 26, 2022
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Ana Lia Anbinder
Grantee:Renata Mendonça Moraes
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Associated scholarship(s):18/21701-0 - Evaluation of the presence and stimulation of sympathetic nervous system in the periodontium, BE.EP.DR

Abstract

In recent decades the negative relationship between stress and periodontal disease has been observed. The literature demonstrates that stress aggravates periodontal disease by activation of the central nervous system with release of cortisol, behavior's alterations, and stimulation of the Sympathetic Nervous System (SNS) with catecholamines release. The SNS has influence on several factors associated with the pathogenesis of periodontal disease as the inflammatory host response, the periodontal resident cells and periodontopathogenic bacteria. Thus, the aim of this study is to evaluate the presence and influence of the SNS in the stimulation of periodontal vasculature, as well as the influence of noradrenaline (hormone released by the SNS) on the expression of virulence factors from Porphyromonas gingivalis (Pg) and on the immunological response of Galleria mellonella. Genetically modified mice labeled by the fluorescent protein e GFP in the SNS fibers will be used to describe the presence of these fibers in the periodontal tissues around the lower first molar region. Isoproterenol (ISO-beta-adrenergic agonist) will also be administered to wild-type mice and the influence of sympathetic activation on angiogenic stimulation in periodontal tissues will be assessed by immunohistochemistry (VEGF-a, VEGFR2, AML and CD34). Finally, the influence of noradrenaline on the virulence factors of Pg and on the immunological response of Galleria mellonella infected by this periodontopathogen will be evaluated through survival curve, hemocyte count, lysozyme test and molecular biology for the evaluation of fimbriae, gingipain, cecropin and gloverin genes. The data will be submitted to appropriate statistical analysis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GOMEZ BAUTISTA, CRISTHIAN REYNALDO; DOS SANTOS, INGRID VALADARES; MORAES, RENATA MENDONCA; CHIBA, FERNANDO YAMAMOTO; SUMIDA, DORIS HISSAKO; DE MORAES, MICHELE BIANCHI; REIS DE VASCONCELLOS, LUANA MAROTTA; ANBINDER, ANA LIA. Sitagliptin's effects on bone tissue and osseointegration in diabetic rats. ARCHIVES OF ORAL BIOLOGY, v. 102, p. 238-243, . (14/20973-6, 17/26461-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MORAES, Renata Mendonça. Stress and periodontitis: evaluation of sympathetic innervation in the periodontium and the influence of adrenergic signaling on Porphyromonas gingivalis infection. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.