Assessment of photodynamic therapy against polymicrobial acne biofilms: a preclinical study

• Grant number: 18/09088-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2018
• Effective date (End): May 31, 2022
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Carla Raquel Fontana Mendonça
• Grantee: Sarah Raquel de Annunzio
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Acne vulgaris is one of the most common dermatological diseases found in clinical practice. The treatment options for this dermatosis are diverse, but they are not always effective. Microbial resistance to antibiotics and the adverse effects of conventional therapies have led researchers to investigate alternative treatments. Phototherapy and more recently photodynamic therapy has been used to inactivate acne-causing bacteria, optimize existing therapies and reduce side effects. In this context, the objective of this study will be divided into three stages. The first one will be the analysis of photodynamic action mediated by curcumin, methylene blue and chlorine-e6 against polymicrobial biofilms formed from samples extracted from patients with moderate and severe acne vulgaris, performing four applications. In the second stage, the biomolecular identification of the collected samples will be made and finally, in the third stage, the cytotoxicity of the proposed treatments will be evaluated in an in vitro model of epidermis. Microbial viability after the proposed treatments will be quantified by colony forming units per milliliter of sample (CFU / mL) and by Confocal Fluorescence Microscopy (CFM). Additionally, this same technique (CFM) will allow the study of the internalization of the photosensitizers and damages in the biofilm matrix. The biomolecular identification of the microorganisms present in the samples will be made by performing qPCR. The toxicity of photodynamic therapy on human epidermis models will be assessed by the Alamar Blue test. Reactive species of intracellular oxygen, production of pro-and anti-inflammatory cytokines and histological analyzes will also be investigated. The results will be evaluated using the most appropriate statistical methods according to each methodology used.

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RICCIO, BRUNO VINCENZO FIOD; DO NASCIMENTO, ANDRE LUIZ CARNEIRO SOARES; MENEGUIN, ANDREIA BAGLIOTTI; RODERO, CAMILA FERNANDA; SANTOS, KAIO PINI; SABIO, RAFAEL MIGUEL; DE ANNUNZIO, SARAH RAQUEL; FONTANA, CARLA RAQUEL; BARUD, HERNANE DA SILVA; FERRARI, PRISCILEILA COLERATO; et al. Solid Dispersions Incorporated into PVP Films for the Controlled Release of Trans-Resveratrol: Development, Physicochemical and In Vitro Characterizations and In Vivo Cutaneous Anti-Inflammatory Evaluation. PHARMACEUTICS, v. 14, n. 6, p. 21-pg., 2022-06-01. (19/19817-3, 18/09088-1, 18/23015-7, 18/25377-3, 18/23357-5, 19/00164-0)

DOS SANTOS, DANILO M.; DE ANNUNZIO, SARAH R.; CARMELLO, JULIANA C.; PAVARINA, ANA C.; FONTANA, CARLA R.; CORREA, DANIEL S.. Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment. ACS APPLIED BIO MATERIALS, DEC 2021. (18/22214-6, 18/23015-7, 17/20973-4, 18/09088-1)

FIORAMONTI CALIXTO, GIOVANA MARIA; DE ANNUNZIO, SARAH RAQUEL; VICTORELLI, FRANCESCA DAMIANI; FRADE, MARIA LUCIA; FERREIRA, PAULA SCANAVEZ; CHORILLI, MARLUS; FONTANA, CARLA RAQUEL. Chitosan-Based Drug Delivery Systems for Optimization of Photodynamic Therapy: a Review. AAPS PHARMSCITECH, v. 20, n. 7, OCT 2019. (18/23015-7, 18/09088-1)

PEREIRA, FELIPE; DE ANNUNZIO, SARAH RAQUEL; LOPES, THAIS DE ASSIS; DE OLIVEIRA, KLEBER THIAGO; CILLI, EDUARDO MAFFUD; BARBUGLI, PAULA ABOUD; FONTANA, CARLA RAQUEL. Efficacy of the combination of P5 peptide and photodynamic therapy mediated by bixin and chlorin-e6 against Cutibacterium acnes biofilm. Photodiagnosis and Photodynamic Therapy, v. 40, p. 13-pg., 2022-12-01. (19/27176-8, 18/09088-1, 19/08668-7, 20/06874-6, 13/07276-1)

SAMPAIO, LAIS SIMOES; DE ANNUNZIO, SARAH RAQUEL; DE FREITAS, LAURA MARISE; DANTAS, LIVIA OLIVEIRA; DE BONI, LEONARDO; DONATONI, MARIA CAROLINA; DE OLIVEIRA, KLEBER THIAGO; FONTANA, CARLA RAQUEL. Influence of light intensity and irradiation mode on methylene blue, chlorin-e6 and curcumin-mediated photodynamic therapy against Enterococcus faecalis. Photodiagnosis and Photodynamic Therapy, v. 31, p. 13-pg., 2020-09-01. (18/23015-7, 18/00106-7, 13/07276-1, 18/09088-1)

DE PAULA ZAGO, LUCAS HENRIQUE; DE ANNUNZIO, SARAH RAQUEL; DE OLIVEIRA, KLEBER THIAGO; BARBUGLI, PAULA ABOUD; VALDES, BELEN RETAMAL; FERES, MAGDA; FONTANA, CARLA RAQUEL. Antimicrobial photodynamic therapy against metronidazole-resistant dental plaque bacteria. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 209, AUG 2020. (16/10310-5, 18/09088-1, 13/07276-1, 18/23015-7)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; DE ANNUNZIO, SARAH RAQUEL; FRANCA, MARIA GLEIZIANE ARAUJO; SILVA, MARIA GORETTI DE VASCONCELOS; CAVALHEIRO, ALBERTO JOSE; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest. PHARMACEUTICS, v. 15, n. 1, p. 16-pg., 2023-01-01. (13/07600-3, 22/12828-2, 14/50926-0, 20/07110-0, 19/08375-0, 18/09088-1, 18/18440-0, 18/23015-7, 21/01191-0)

DOS SANTOS, DANILO M.; CHAGAS, PAULO A. M.; LEITE, ILAIALI S.; INADA, NATALIA M.; DE ANNUNZIO, SARAH R.; FONTANA, CARLA R.; CAMPANA-FILHO, SERGIO P.; CORREA, DANIEL S.. Core-sheath nanostructured chitosan-based nonwovens as a potential drug delivery system for periodontitis treatment. International Journal of Biological Macromolecules, v. 142, p. 521-534, JAN 1 2020. (17/20973-4, 18/23015-7, 18/09088-1, 13/07276-1, 17/12174-4)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; MARQUES, RAQUEL SOUZA; DE ANNUNZIO, SARAH RAQUEL; BRIGHENTI, FERNANDA LOURENCAO; FONTANA, CARLA RAQUEL. Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 14, JUL 2 2019. (18/23015-7, 18/18440-0, 18/09088-1)

LORDELLO, VIRGINIA BARRETO; MENEGUIN, ANDREIA BAGLIOTTI; DE ANNUNZIO, SARAH RAQUEL; PIA TARANTO, MARIA; CHORILLI, MARLUS; FONTANA, CARLA RAQUEL; UMBELINO CAVALLINI, DANIELA CARDOSO. Orodispersible Film Loaded with Enterococcus faecium CRL183 Presents Anti-Candida albicans Biofilm Activity In Vitro. PHARMACEUTICS, v. 13, n. 7, JUL 2021. (18/09088-1, 18/23015-7)

POLLI SILVESTRE, AMANDA LETICIA; DI FILIPPO, LEONARDO DELELLO; BESEGATO, JOAO FELIPE; DE ANNUNZIO, SARAH RAQUEL; FURQUIM DE CAMARGO, BRUNA ALMEIDA; GOBBO DE MELO, PRISCILA BORGES; DE SOUZA RASTELLI, ALESSANDRA NARA; FONTANA, CARLA RAQUEL; CHORILLI, MARLUS. Current applications of drug delivery nanosystems associated with antimicrobial photodynamic therapy for oral infections. International Journal of Pharmaceutics, v. 592, JAN 5 2021. (18/23015-7, 18/23096-7, 18/18488-3, 18/09088-1)

DE SANTANA, WILLIAN MAX O. S.; CAETANO, BRUNO L.; DE ANNUNZIO, SARAH RAQUEL; PULCINELLI, SANDRA H.; MENAGER, CHRISTINE; FONTANA, CARLA RAQUEL; SANTILLI, V, CELSO. Conjugation of superparamagnetic iron oxide nanoparticles and curcumin photosensitizer to assist in photodynamic therapy. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 196, DEC 2020. (18/23015-7, 18/09088-1, 17/07822-7)

DE ANNUNZIO, SARAH RAQUEL; SILVA COSTA, NATALIA CAROLINE; MEZZINA, RAFAELA DALBELLO; GRAMINHA, MARCIA A. S.; FONTANA, CARLA RAQUEL. Chlorin, Phthalocyanine, and Porphyrin Types Derivatives in Phototreatment of Cutaneous Manifestations: A Review. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 16, AUG 2 2019. (18/23015-7, 17/03552-5, 18/09088-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ANNUNZIO, Sarah Raquel de. Evaluation of photodynamic therapy against polymicrobial acne biofilms: a preclinical study. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.