Acne vulgaris is one of the most common dermatological diseases found in clinical practice. The treatment options for this dermatosis are diverse, but they are not always effective. Microbial resistance to antibiotics and the adverse effects of conventional therapies have led researchers to investigate alternative treatments. Phototherapy and more recently photodynamic therapy has been used to inactivate acne-causing bacteria, optimize existing therapies and reduce side effects. In this context, the objective of this study will be divided into three stages. The first one will be the analysis of photodynamic action mediated by curcumin, methylene blue and chlorine-e6 against polymicrobial biofilms formed from samples extracted from patients with moderate and severe acne vulgaris, performing four applications. In the second stage, the biomolecular identification of the collected samples will be made and finally, in the third stage, the cytotoxicity of the proposed treatments will be evaluated in an in vitro model of epidermis. Microbial viability after the proposed treatments will be quantified by colony forming units per milliliter of sample (CFU / mL) and by Confocal Fluorescence Microscopy (CFM). Additionally, this same technique (CFM) will allow the study of the internalization of the photosensitizers and damages in the biofilm matrix. The biomolecular identification of the microorganisms present in the samples will be made by performing qPCR. The toxicity of photodynamic therapy on human epidermis models will be assessed by the Alamar Blue test. Reactive species of intracellular oxygen, production of pro-and anti-inflammatory cytokines and histological analyzes will also be investigated. The results will be evaluated using the most appropriate statistical methods according to each methodology used.
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