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Regulation of the SMyHC iii promoter by the nuclear receptors AR, GR and Coup-TFII: a solo or joint work?

Grant number: 18/02481-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2018
Effective date (End): May 31, 2020
Field of knowledge:Biological Sciences - Biophysics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Ana Carolina Migliorini Figueira
Grantee:Izabella Luisa Tambones
Home Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia, Inovações e Comunicações (Brasil). Campinas , SP, Brazil
Associated scholarship(s):19/13928-8 - Biophysical and structural characterization of the GR/COUP-TFII complex, BE.EP.MS

Abstract

Nuclear Receptors (NRs) correspond to a superfamily of ligand modulated transcription factors that act in the regulation of specific genes. The orphan receptor, Coup-TFII (Chiken ovalbumin upstream promoter transcription factor) has been suggested as one of the key factors involved in cardiac development, controlling slow myosin heavy chain (SMyHC III) gene. The promoter involved in the atrial differentiation and activates the SMyHC III gene, owns a "complex 33-base pair nuclear receptor response element" (CNRRE) sequence, which containing probable binding sites for NRs, such as Coup -TF II. Transient transfection studies performed by our research group revealed Coup-TFII activation on the SmyHC III promoter, which specifically binds to CNRRE. In addition, previous studies have shown differences in this promoter activation under different combinations of androgens receptor (AR) and glucocorticoids receptor (GR) with Coup-TFII, suggesting a possible cooperation between Coup-TFII, GR and AR. Therefore, this study aims to evaluate, in vitro, protein-protein and protein-DNA interactions involving the AR, GRs and COUP-TFII and the possible relationship between their roles in the atrial development via SMyHC III regulation. The determination of protein-protein and DNA-protein affinities will be performed using fluorescence anisotropy and microscale thermophoresis. Thus, the obtained results through this present work may contribute to the elucidation of regulatory pathways, which act directly or indirectly in the biological process of atrial cardiac differentiation. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO FILHO, HELDER VERAS; TAMBONES, IZABELLA LUISA; GONCALVES DIAS, MARIELI MARIANO; VIDEIRA, NATALIA BERNARDI; BRUDER, MARJORIE; AMATO, ANGELICA AMORIM; MIGLIORINI FIGUEIRA, ANA CAROLINA. Modulation of nuclear receptor function: Targeting the protein-DNA interface. Molecular and Cellular Endocrinology, v. 484, p. 1-14, MAR 15 2019. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.