Regulation of the SMyHC iii promoter by the nuclear receptors AR, GR and Coup-TFII: a solo or joint work?

• Grant number: 18/02481-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2018
• End date: July 31, 2020
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ana Carolina Migliorini Figueira
• Grantee: Izabella Luisa Tambones
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Campinas , SP, Brazil
• Associated scholarship(s): 19/13928-8 - Biophysical and structural characterization of the GR/COUP-TFII complex, BE.EP.MS

Abstract

Nuclear Receptors (NRs) correspond to a superfamily of ligand modulated transcription factors that act in the regulation of specific genes. The orphan receptor, Coup-TFII (Chiken ovalbumin upstream promoter transcription factor) has been suggested as one of the key factors involved in cardiac development, controlling slow myosin heavy chain (SMyHC III) gene. The promoter involved in the atrial differentiation and activates the SMyHC III gene, owns a "complex 33-base pair nuclear receptor response element" (CNRRE) sequence, which containing probable binding sites for NRs, such as Coup -TF II. Transient transfection studies performed by our research group revealed Coup-TFII activation on the SmyHC III promoter, which specifically binds to CNRRE. In addition, previous studies have shown differences in this promoter activation under different combinations of androgens receptor (AR) and glucocorticoids receptor (GR) with Coup-TFII, suggesting a possible cooperation between Coup-TFII, GR and AR. Therefore, this study aims to evaluate, in vitro, protein-protein and protein-DNA interactions involving the AR, GRs and COUP-TFII and the possible relationship between their roles in the atrial development via SMyHC III regulation. The determination of protein-protein and DNA-protein affinities will be performed using fluorescence anisotropy and microscale thermophoresis. Thus, the obtained results through this present work may contribute to the elucidation of regulatory pathways, which act directly or indirectly in the biological process of atrial cardiac differentiation. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

RIBEIRO FILHO, HELDER VERAS; TAMBONES, IZABELLA LUISA; GONCALVES DIAS, MARIELI MARIANO; VIDEIRA, NATALIA BERNARDI; BRUDER, MARJORIE; AMATO, ANGELICA AMORIM; MIGLIORINI FIGUEIRA, ANA CAROLINA. Modulation of nuclear receptor function: Targeting the protein-DNA interface. Molecular and Cellular Endocrinology, v. 484, p. 1-14, MAR 15 2019. (16/22246-0, 18/02481-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TAMBONES, Izabella Luisa. Regulation of the SMyHC3 promoter by the nuclear receptors GR and COUP-TFII: a solo or cooperative work?. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.