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Lyophilized PRP associated with nano-hydroxyapatite in cell performance and bone regeneration

Grant number: 18/05731-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2018
Effective date (End): January 31, 2023
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Willian Fernando Zambuzzi
Grantee:Marcel Rodrigues Ferreira
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration, AP.JP
Associated scholarship(s):21/14271-2 - Platelet-Rich Plasma and additive manufacturing as a promising alternative to help grow real bone, BE.EP.DR


Bone lesions are one of the most pressing problems in the medical and dental area. Although bone tissue is a highly dynamic tissue with good regeneration capacity, in the case of more severe lesions it is necessary to use biomaterials that allow a good tissue regeneration. To date, no synthetic material has been developed that combines the properties and capabilities offered by the autogenous material, known as the ideal material. Among the several therapies proposed, the association of biomaterials on a nanometric scale, such as hydroxyapatite, has been showing positive results in the regeneration process of bone tissue. Another widely used aspect is the use of platelet rich plasma (PRP), obtained from the patient himself, in the treatment of these lesions. Based on this previous knowledge about the potential of both therapies, the objective of this project is the association of hydroxyapatite at nano-scale (nanoHA) with lyophilized PRP to obtain a product capable of impacting bone regeneration and therefore the quality of life of the patient. We will use the previous experience of our group for a characterization of the impact of this composite in classic signaling pathways related to osteoblast differentiation processes and bone tissue regeneration through in vitro and in vivo assay, added to the experience of Prof. Elenice Deffune, FMB-UNESP, to obtain legal and PRP processing. We would like to point out that we are interested in requesting, in a timely manner, the complementary scholarship BEPE, for the stage in the United States. Through the partnership with Prof. Paulo G. Coelho, from New York University (New York - USA), will try to fabricate, using 3D printers, prosthesis, using the proposed composite. We also aimed to evaluate the effects of this product on critically sized defects in rat skull cap. Although the theme of this project was well explored in the literature, our motivation is precisely to know the cellular and molecular aspects that govern its performance during regeneration mechanisms, besides proposing previous protocols of synthesis / combinations, as an endpoint to propose new methodological alternatives to clinical practice, facilitating its application in injuries of the bone tissue, supported by a molecular repertoire that justifies its choice and performance, parameters extremely necessary to interface with the productive sector. Finally, we intend that this new routine can agglutinate impact information in scientific articles and patents, as well as the acquisition of knowledge about related methodologies, not yet practiced in the Dep. Chemistry and Biochemistry, IBB-UNESP, through human resources qualification. The proposed project will use funding acquired through JP-FAPESP (2014 / 22689-3).

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA, MARCEL R.; SANTOS, GERSON A.; BIAGI, CARLOS A.; SILVA JUNIOR, WILSON A.; ZAMBUZZI, WILLIAN F.. GSVAscore reveals molecular signatures from transcriptomes for biomaterials comparison. Journal of Biomedical Materials Research Part A, v. 109, n. 6, . (14/22689-3, 18/05731-7, 15/03639-8, 13/08135-2)
FERREIRA, MARCEL RODRIGUES; ZAMBUZZI, WILLIAN FERNANDO. Platelet microparticles load a repertory of miRNAs programmed to drive osteogenic phenotype. Journal of Biomedical Materials Research Part A, v. 109, n. 8, p. 1502-1511, . (14/22689-3, 19/26854-2, 18/05731-7)
BERGAMO, EDMARA T. P.; BALDERRAMA, ISIS DE FATIMA; FERREIRA, MARCEL RODRIGUES; SPIELMAN, ROBERT; SLAVIN, BLAIRE V.; TORRONI, ANDREA; TOVAR, NICK; NAYAK, VASUDEV V.; SLAVIN, BENJAMIN R.; COELHO, PAULO G.; et al. Osteogenic differentiation and reconstruction of mandible defects using a novel resorbable membrane: An in vitro and in vivo experimental study. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 111, n. 11, p. 13-pg., . (21/08018-2, 18/05731-7, 19/08693-1, 21/14557-3, 21/14271-2)
FERREIRA, MARCEL RODRIGUES; MILANI, RENATO; RANGEL, ELIDIANE C.; PEPPELENBOSCH, MAIKEL; ZAMBUZZI, WILLIAN. OsteoBLAST: Computational Routine of Global Molecular Analysis Applied to Biomaterials Development. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, . (18/05731-7, 14/22689-3, 15/03639-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FERREIRA, Marcel Rodrigues. Lyophilized platelet-rich plasma associated with nanohydroxyapatite in cell performance and bone regeneration. 2023. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

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