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Mechanisms of omega-3 fatty acid -induced immune response in cows by in vitro cell culture and metabolic functions by in vivo supplementation

Grant number: 18/16051-7
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): October 03, 2018
Effective date (End): July 31, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Eneiva Carla Carvalho Celeghini
Grantee:Luisa Cunha Carneiro
Supervisor abroad: Erin Jane Williams
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Edinburgh, Scotland  
Associated to the scholarship:17/03622-3 - Effects of OMEGA- 3 supplementation on reproductive performance and its immune response in dairy cows, BP.PD


Complementary to the current Post-doctoral FAPESP fellowship (2017/03622-3) we herein present a proposal for an abroad research internship at the Royal (Dick) School of Veterinary Studies. The main of the study is twofold: first, to determine whether Omega-3 polyunsaturated fatty acids modulate bovine endometrial cell function in vitro; second, to determine whether dietary supplementation of Omega-3 polyunsaturated fatty acids results in alteration of immune, metabolic and health parameters in dairy cattle. In the in vitro studies, uterine tissue will be obtained from a local abattoir and epithelial and stromal cell populations will be isolated. Cells will be challenged with bacterial LPS and cultured in the presence or absence of fatty acids and outputs will be monitored. It will be determined the expression of key immune genes in the Toll Like Receptor-4 (TLR4) pathway and expression and production of inflammatory cytokines IL-1, IL-6 and IL-8 to determine whether Omega-3 fatty acids modulate the uterine inflammatory response to pathogenic stimuli. In vivo, Holstein-Friesian dairy cattle will be supplemented with Omega-3 during the peripartum period. Blood samples will be collected across the experimental period to monitor their immune and metabolic status; cytokines and acute phase proteins will be measured as well as metabolic indicators. Uterine health will be monitored in the postpartum period and analysis of the microbiome will be completed to determine whether supplementation improves the uterine response to bacterial dysbiosis in the postpartum period. In summary, the project addresses a missing link in the original project, which will allow us to compare the effects of supplementation in in vitro bovine cells versus in vivo response.