Advanced search
Start date
Betweenand

Dental pulp stem cell biology

Grant number: 18/13675-0
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 01, 2018
Effective date (End): September 30, 2019
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Thais Marchini de Oliveira Valarelli
Grantee:Mariel Tavares de Oliveira Prado Bergamo
Supervisor: Jacques Eduardo Nor
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Research place: University of Michigan, United States  
Associated to the scholarship:17/11396-3 - Protein gene expression by stem cells from pulp of deciduous teeth after the use of low intensity laser, BP.DR

Abstract

This study aims to evaluate the viability and proliferation of Stem Cell from Human Exfoliated Teeth (SHED); mRNA gene expression and protein synthesis of DMP-1, DSPP, VEGF, and FGF2 after photobiomodulation; and SHED differentiation into endothelial cells through VEGF expression. The characterization of the SHED will be performed with hMSC Analysis Kit with flow cytometry. The groups will be divided according to the energy densities. The techniques used for the analysis will be MTT, Crystal Violet, RT-PCR, ELISA, Immunofluorescence, Western Blot, Capillary Sprouting Assay, Tooth Slices/Scaffold, and RGB Assay. The values in triplicate will be analysed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test respecting normal and non-normal distribution, respectively. The level of significance of 5% will be adopted for statistically significant differences. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERGAMO, M. T.; ZHANG, Z.; OLIVEIRA, T. M.; NOR, J. E.. VEGFR1PRIMES A UNIQUE COHORT OF DENTAL PULP STEM CELLS FOR VASCULOGENIC DIFFERENTIATION. EUROPEAN CELLS & MATERIALS, v. 41, p. 332-344, . (18/13675-0)

Please report errors in scientific publications list using this form.