Wound healing is a process characterized in three sequential and overlapping phases, which involve: inflammation, proliferation and maturation. The inflammatory response is determinant for the onset of the subsequent phase, in which the extracellular matrix (ECM) will be produced. The MEC is a three-dimensional structure that provides support, resistance, tension and organization to the tissues, besides promoting the adhesion of the cells with their components, facilitating cellular communication. Recently, we have demonstrated that FAT-1 mice, capable of producing endogenous É-3 fatty acids, delay the healing of cutaneous wounds. In these mice, there was an increase in the production of pro-inflammatory mediators (IL-6, CXCL1 and CXCL2) and reduction of IL-10. Additionally, by PCR array assay, the FAT-1 mice modulated the expression of several genes essential to the structure and function of ECM. Thus, in the present work, we intend to investigate the effects of the inflammatory response on the ECM composition, in the context of the healing of cutaneous wounds in FAT-1 mice. To do so, we will carry out immunophenotyping tests to characterize the main cellular populations present during skin repair. From these results, we will temporarily evaluate the gene and / or protein expression of the ECM components, such as laminin, fibronectin, collagen I and III, hyaluronic acid and decorin. We will use bone marrow transplantation to determine if the effects observed are due to the actions of myeloid cells. We will then investigate the influence of the inflammatory response on the formation of ECM by depleting the macrophages of mice supplemented with fish oil and evaluate the expression of ECM components as well as the production of cytokines and growth factors in the scar tissue of these animals.
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