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Influence of the inflammatory response on the extracellular matrix composition during the wound healing in FAT-1 mice

Grant number: 17/26366-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2018
Effective date (End): June 30, 2021
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal researcher:Hosana Gomes Rodrigues
Grantee:Thamiris Candreva Robles
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Associated research grant:13/06810-4 - Mechanisms of action of omega-3 and omega-6 in the tissue repair process: neuro-immune focus, AP.JP


Wound healing is a process characterized in three sequential and overlapping phases, which involve: inflammation, proliferation and maturation. The inflammatory response is determinant for the onset of the subsequent phase, in which the extracellular matrix (ECM) will be produced. The MEC is a three-dimensional structure that provides support, resistance, tension and organization to the tissues, besides promoting the adhesion of the cells with their components, facilitating cellular communication. Recently, we have demonstrated that FAT-1 mice, capable of producing endogenous É-3 fatty acids, delay the healing of cutaneous wounds. In these mice, there was an increase in the production of pro-inflammatory mediators (IL-6, CXCL1 and CXCL2) and reduction of IL-10. Additionally, by PCR array assay, the FAT-1 mice modulated the expression of several genes essential to the structure and function of ECM. Thus, in the present work, we intend to investigate the effects of the inflammatory response on the ECM composition, in the context of the healing of cutaneous wounds in FAT-1 mice. To do so, we will carry out immunophenotyping tests to characterize the main cellular populations present during skin repair. From these results, we will temporarily evaluate the gene and / or protein expression of the ECM components, such as laminin, fibronectin, collagen I and III, hyaluronic acid and decorin. We will use bone marrow transplantation to determine if the effects observed are due to the actions of myeloid cells. We will then investigate the influence of the inflammatory response on the formation of ECM by depleting the macrophages of mice supplemented with fish oil and evaluate the expression of ECM components as well as the production of cytokines and growth factors in the scar tissue of these animals.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; et al. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, . (17/25679-7, 12/10653-9, 17/01451-7, 15/06134-4, 16/23142-3, 17/26366-2, 17/06577-9, 17/16280-3, 18/02208-1, 13/06810-4, 14/03002-7, 18/01753-6, 15/01507-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ROBLES, Thamiris Candreva. Influence of the inflammatory response on the composition of the extracellular matrix during the wound healing in FAT-1 mice. 2021. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.

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